Cell Preparation:
http://media.ebioscience.com/data/pdf/best-protocols/cell-preparation-for-flow-cytometry.pdf
Annexin V:http://media.ebioscience.com/data/pdf/best-protocols/annexin-v-staining.pdf(This shows with PI/7-AAF and fixable viability dyes)Viability Staining:http://media.ebioscience.com/data/pdf/best-protocols/viability-staining.pdf(Includes PI/7-AAD and Fixable Dyes. eBioscience Specific)Cell surface staining:http://media.ebioscience.com/data/pdf/best-protocols/staining-cell-surface-antigens-for-flow-cytometry.pdfIntracellular and Intranuclear Staining:http://media.ebioscience.com/data/pdf/best-protocols/staining-intracellular-antigens-for-flow-cytometry.pdfProliferation:http://media.ebioscience.com/data/pdf/best-protocols/proliferatione-dyes-for-flow-cytometry.pdf(covers a couple eFluor dyes as well as CFSE; should double check that the CFSE concentration is consistent with our SOP)
Tissue Dissociation Guide: http://www.worthington-biochem.com/tissuedissociation/default.html
http://www.maeckerlab.typepad.com/
Minimum information about a flow cytometry experiment (MiFlowCyt)
Minimal Information About T cell Assays (MIATA)
MIATA Easy Reporting of Experiments
Macrophage activation and polarization: nomenclature and experimental guidelines
BD fluorescence spectrum viewer
Amine-reactive dyes for dead cell discrimination in fixed samples (Perfetto et al., 2010)
BD compensation multicolor technical bulletin (2009)
Optimizing a multi-color immunophenotyping assay (Mahnke & Roederer, 2007)
A very helpful tool to appreciate absolute cell numbers to be expected is the Frequencies of Cell Types chart published by StemCell Technologies.