Useful Links

Cell Preparation:

Annexin V: shows with PI/7-AAF and fixable viability dyes)Viability Staining: PI/7-AAD and Fixable Dyes. eBioscience Specific)Cell surface staining: and Intranuclear Staining: a couple eFluor dyes as well as CFSE; should double check that the CFSE concentration is consistent with our SOP)

Tissue Dissociation Guide:

How many events are enough?

Minimum information about a flow cytometry experiment (MiFlowCyt)

Minimal Information About T cell Assays (MIATA)

MIATA checklist

MIATA Easy Reporting of Experiments

Macrophage activation and polarization: nomenclature and experimental guidelines

BD fluorescence spectrum viewer

Amine-reactive dyes for dead cell discrimination in fixed samples (Perfetto et al., 2010)

BD compensation multicolor technical bulletin (2009)

Optimizing a multi-color immunophenotyping assay (Mahnke & Roederer, 2007)

A very helpful tool to appreciate absolute cell numbers to be expected is the Frequencies of Cell Types chart published by StemCell Technologies.