|FACSAria IIIu (assisted use only)||$125/h||$275/h|
|Sony SH-800 (unassisted use)||$65/h||$180/h|
|All other cytometers (assisted use)||$115/h||$250/h|
|All other cytometers (unassisted)||$55/h||$180/h|
|Immune Monitoring Services||Dartmouth||External|
|PBMC isolation + cryopreservation||$400/20 ml WB*||$1000/20 ml WB*|
|plasma/serum/CSF isolation + cryopreservation||$60/sample||$150/sample|
|Procurement of WB||$60/10ml||$150/10ml|
|Luminex assay (includes analysis)||$564 + kit||$1700 (kit supplied)|
|ELISAs (includes analysis)||$400 + kit||$800 + kit|
|ELISPOT plate reading||$50/plate||$100/plate|
|tumor isolation||contact us||contact us|
|* WB = whole blood|
Analysis time is free for internal users, and negotiable for external users. The lab has a computing area (Rubin 720) with Macintosh workstations with oversized dual monitors. If you want to use a computer attached to a cytometer, acquisition of new data on the instruments has priority. For this reason, analysis time on computers attached to the instruments cannot be reserved in advance. Booking schedules are on-line in RaDar. Users are charged for time used and for time booked (even if it has not been used). Cancelled time will be charged unless it is cancelled 24 hours in advance and the cancellation has been confirmed with lab personnel. The sign-up schedule for the instruments is used (i) to reserve time, (ii) as accounting records for billing purposes, and (iii) for communication between users (e.g. Do I need to shut down the instrument or is someone following me at midnight? Will someone have five minutes to spare sometime during the day?). Users of the FACSAria need to confirm with Gary Ward about his availability for operation of the instrument before booking. It is expected that users will be trained to run the flow cytometers and will then use those instruments without direct supervision. Training sessions and help for new users are provided free of charge.
DartLab welcomes the opportunity to collaborate in development of new methodologies; there is no charge for development work or feasibility studies. Internal use of the Shared Resource is subsidized by the NCI Cancer Center Core Grant. External users that are part of the New Hampshire INBRE program will pay internal rates.