Before you decide on a fluorochrome for a flow cytometric assay, you need to know:
- the wavelengths of the lasers in the cytometer
- the wavelengths of the filters in front of the photomultiplier tubes (PMTs) in the cytometer
- the absorption and fluorescence wavelengths of fluorochromes to be used
These can be simulated using the BD fluorescence spectrum viewer
Gallios Optical Bench
Violet laser configuration:
Blue laser configuration:
Red laser configuration:
Gallios video from Beckman Coulter
Stain index
