10-color Gallios (710 Rubin)

Before you decide on a fluorochrome for a flow cytometric assay, you need to know:

  • the wavelengths of the lasers in the cytometer
  • the wavelengths of the filters in front of the photomultiplier tubes (PMTs) in the cytometer
  • the absorption and fluorescence wavelengths of fluorochromes to be used

These can be simulated using the BD fluorescence spectrum viewer

Gallios Optical Bench

Violet laser configuration:

Blue laser configuration:

Red laser configuration:

Gallios red laser filters

Gallios video from Beckman Coulter

Stain index

Click here for Gallios Specifications.