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CytoFLEX (2 Remsen)

The CytoFLEX (Beckman Coulter) is a four laser, 15 detector analysis flow cytometer capable of measuring 13 colors simultaneously. The lasers in this instrument are Violet (405nm), Blue (488nm), Yellow-Green (561nm), and Red (638nm).  The Wavelength Division Multiplexer (WDM) deconstructs and measures multiple wavelengths of light, relying on fiber optics and band pass filters to separate the light wavelengths. The WDM utilizes Avalanche photodiode detectors versus photomultiplier tubes, resulting in a high quantum efficiency in excess of 80%, especially for wavelengths greater than 800 nm.  The CytoFLEX also has a high throughput 96 well plate loader.

 

Location
(BSL2)
Remsen, room 243C Book the CytoFLEX
Rates  Dartmouth: Self-operated $50 per hour
Pharma: Self-operated $160 per hour/Assisted $180 per hour plus $180 set-up feeBilling policy: Users are billed for the  greater of time reserved or actual time used on the instrument.
Cancellation policy: Users must cancel their reservations 24 hours in advance of their scheduled appointment to avoid  charges.
Hours  24 hours / 7 days a week
PMTs  Violet: 450/45, 525/40, 610/20, 660/20

Blue: SSC, 525/40, 690/50

Yellow: 561/10, 585/42, 610/20 (mCherry), 690/50, 780/60

Red: 660/20, 712/25, 780/60

 

Online trainings and tutorials:

-            Log on to the Beckman Coulter Learning Center using the following link:  https://beckmancoulter.csod.com/client/beckmancoulter/default.aspx

-            Select “New user Register” (at the bottom of the page) and create an account

-            On the main page, top right corner, select the search field and type “animated”. This will give you access to a series of five videos on the basic principles of flow cytometry.

-            On the main page, top right corner, select the search field and type “CytoFLEX”. Choose the videos called “CytoFLEX LX Basic operator online training-single tube” and “CytoFLEX LX Basic operator online training-plate loader”. These videos give an overview of the basic operation of the CytoFLEX.

Book: “Practical Flow Cytometry”, 4th Edition, Howard Shapiro

Video for small particle detection: “Extracellular vesicle detection and analysis via flow cytometry”, Vasilis Toxavidis and John Tigges: https://www.youtube.com/watch?v=csnPPY-8FZk