Before You Sort
All cell sorting experiments on the Aria are operator-assisted. Before getting started with sorting, you will need to fill out the following online pre-sort questionnaire:
To schedule a sort, please book the time of your sort on RaDar and also send an email to Gary Ward (email@example.com) and cc' Mikayla Kravetz-Hernandez (firstname.lastname@example.org).
Staining panels for cell sorting must be optimized to allow for clear discrimination of populations of interest. The following optical bench information is necessary for designing a panel for a FACS experiment. We also recommend that you consult with DartLab staff prior to staining your sample for any pilot sort.
Sorting Recovery Calculations
The following spreadsheet can be used to calculate cell recovery following an Aria sort: