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Robert A. Maue, Ph.D.

Title(s):
Professor of Physiology
Professor of Biochemistry
Adjunct Professor of Psychological Brain Sciences
Adjunct Professor of Biological Sciences

Department(s):
Physiology
Biochemistry

Education:
U. California - San Diego, PHD 1985

Programs:
Academy of Master Faculty Educators
Molecular and Cellular Biology Graduate Programs
Neuroscience Center at Dartmouth
Program in Experimental and Molecular Medicine

Websites:
http://dms.dartmouth.edu/ncd/

Contact Information:

Geisel School of Medicine
HB 7701
Hanover NH 03755

Office: Remsen 210
Phone: 603-650-1726/650-1119
Fax: 603-650-1128
Email: robert.maue@dartmouth.edu


Professional Interests:

We are interested in understanding the mechanisms important for the development and differentiation of neurons in the brain. In particular, we are interested in the means by which a family of growth factors known as neurotrophins (NGF, BDNF, NT-3) exert their influence the growth and electrical activity of neurons, including their regulation of process outgrowth and ion channel expression. Our recent efforts have focused on a class of neurons in the brain known as cerebellar Purkinje cells, and our interest in events associated with their development has recently focused on the pathology and abnormal development of these neurons in neurological disorders such as Niemann Pick Type C (NPC) disease. NPC disease is a fatal genetic disorder associated with abnormal cellular cholesterol accumulation, and a hallmark of this disorder is the preferential loss of Purkinje cells. Given the recent appreciation of the importance of cholesterol in the brain, particularly during neurodegenerative diseases such as NPC disease, Alzheimer's disease, and Huntington’s disease, understanding the effects of NPC disease and cholesterol abnormalities on Purkinje cells has widespread implications.

In our experiments we have taken advantage of transgenic mice expressing mutant ion channel genes, knockout mice lacking neurotrophin receptors, and novel mouse models of NPC disease that we've developed to analyze neurons in primary culture, in brain slices, and in vivo. This has included immunocytochemical and morphometric analyses, Western blotting and biochemical assays, single-cell, real time PCR analyses of gene expression, patch clamp recording of ion channels and electrical activity, virus-mediated expression of fluorescent proteins in vivo and in cultured neurons, and behavioral analyses of cerebellar function.

Grant Information:

National Institutes of Health, National Niemann Pick Disease Foundation, Ara Parseghian Medical Research Foundation, Hereditary Disease Foundation, Epilepsy Foundation, Wellcome Trust

Courses Taught:

-Instructor Medical Neuroscience (Medical student course) 1990-present

-Instructor PEMM Core Course (Grad course PEMM Program) 2006-2013

-Director/Lecturer Adv Biomed Sci (Grad course PEMM Program) 2008-2010

-Instructor Molec Cell Biol (Grad core course - MCB Program) 1999-2003

-Instructor Receptor Pharm (Grad course -– Pharmacology Program) 1996-2006

-Director/Instructor Stystems Neuroscience course (PBS 65 upper div undergrad course) 2010-present

-Director/Instruct Cellular/ Molec Neuro course (PBS 46 upper div undergrad course) 2007-present

-Director/Instruct Physiol (Bio 14 upper division undergrad course) 2008- present

-Director/Instruct Physiology (Bio 35 upper division undergrad course) 2006

-Director/Instruct Adv Neurobiology (Bio 74 upper div undergrad course) 2006

-Instructor Biochemistry (Bio 78 upper division undergrad course) 1998-2005

-Instructor Intro Cell Biology (Bio 15 upper division undergrad course) 2003

Biography:

Bob received a B.S. in Biology from St John’s University in Minnesota, a Ph.D. in Physiology and Pharmacology from the University of California - San Diego (including two research expeditions to Antarctica), and did postdoctoral research at Brandeis University in Waltham and at New England Medical Center in Boston before becoming an Assistant Professor at Dartmouth in 1989. He is currently a Professor in the Department of Physiology and Neurobiology and the Department of Biochemistry in the Geisel School of Medicine, and an Adjunct Professor in the Department of Psychological Brain Sciences and the Department of Biological Sciences at Dartmouth College.

Bob is a cellular and molecular neurobiologist with research interests in neuronal development, neurotrophic factors, electrical excitability, and developmental aspects of neurodegenerative diseases, and was the first Dartmouth faculty member to receive a coveted Sloan Research Fellowship. Support for his research has also come from the National Institutes of Health (NIH) as well as the Epilepsy Foundation, Hereditary Disease Foundation, National Neimann Pick Disease Foundation, and Ara Parseghian Medical Research Foundation. He has served on and chaired numerous scientific advisory boards and grant review panels for NIH and the National Science Foundation (NSF), including the NSF review panel for course, curriculum, and lab implementation (CCLI) proposals.

Bob is passionate about teaching, and in the past 25 years has taught a broad spectrum of medical students, graduate students, undergraduate students, and high school students in both the classroom and a variety of laboratory settings. This includes extensive teaching experience at Dartmouth College, where by invitation he has served as the course director for a systems neuroscience course and lab (2010-present), for a physiology course and lab (2009-present), a molecular and cellular neuroscience course (2007-present), and an advanced neurobiology course (2006). He has mentored more than two dozen students in their Honors Thesis Research, including recipients of the “Christopher Reed Biologist Award for distinguished biological research”. He has served as the founder and co-director of a summer undergraduate nursing research program at Dartmouth Hitchcock Medical Center (2010-present), as a member of the steering committee for the undergraduate neuroscience major (2010-present), and as a member of the committee on undergraduate research (CUGR) at Dartmouth (2013-present). In 2013 he was one of two nominees from Dartmouth College for New Hampshire Professor of the Year. Beyond the confines of Dartmouth, Bob has taught at Hanover High School (2005-present) and Stevens High School (2010), as well as teaching and helping design teaching activities at River Valley Community College (2011-present). In the past several years (2010-present) a major focus has been serving as a leader of the New Hampshire IdeA Network for Biomedical Research Excellence (NH-INBRE), a large NIH-supported initiative for developing research and science education opportunities for students at primarily undergraduate institutions (PUIs) across the state of New Hampshire.

At the graduate student level, Bob is a member of both the Molecular and Cellular Biology (MCB) graduate program and the graduate Program in Experimental and Molecular Medicine (PEMM). As such he has served as a lecturer in MCB graduate student core course (1999-2003), course director and lecturer in the PEMM graduate student core course (2006-2013), and as a lecturer in the PEMM 211 and PEMM 271 graduate courses (2008-2010). He has trained nearly a dozen MD, PhD, and MD/PhD students in his lab, including recipients of the “Strohbehn Medal for Excellence in Biomedical Research”. He has served as Co-Director of the prestigious “Ion Channels” summer course at Cold Spring Harbor Laboratory (1996-1998), and twice has served as a course faculty member in the Woods Hole summer Neurobiology course (1988; 2004). At the Geisel School of Medicine, Bob has been a fixture in the Medical Neuroscience course (1990-present) and for his efforts has been nominated for the Excellence in Education Award on numerous occasions (2010, 2011, 2012). He is involved in the medical curriculum redesign, and serves on several Redesign Task Force committees (2011-present).

Bob is currently mentoring faculty in the design of effective presentations, the design of student lab activities and inquiry-based experimental exercises, course development and organization, teaching in small and large group environments, and student mentoring.


Selected Publications:

 

  • Paul CA, Boegle AK, Maue RA. Before the loss: neuronal dysfunction in Niemann-Pick Type C disease. Biochim Biophys Acta. 2004 Oct 11;1685(1-3):63-76. Review. (view details on MedLine)

  • Paul CA, Reid PC, Boegle AK, Karten B, Zhang M, Jiang ZG, Franz D, Lin L, Chang TY, Vance JE, Blanchette-Mackie J, Maue RA. Adenovirus expressing an NPC1-GFP fusion gene corrects neuronal and nonneuronal defects associated with Niemann pick type C disease. J Neurosci Res. 2005 Sep 1;81(5):706-19. (view details on MedLine)

  • Fry M, Boegle AK, Maue RA. Differentiated pattern of sodium channel expression in dissociated Purkinje neurons maintained in long-term culture. J Neurochem. 2007 May;101(3):737-48. (view details on MedLine)

  • Maue RA. Understanding ion channel biology using epitope tags: progress, pitfalls, and promise. J Cell Physiol. 2007 Dec;213(3):618-25. Review. (view details on MedLine)

  • Claudepierre T, Paques M, Simonutti M, Buard I, Sahel J, Maue RA, Picaud S, Pfrieger FW. Lack of Niemann-Pick type C1 induces age-related degeneration in the mouse retina. Mol Cell Neurosci. 2010 Jan;43(1):164-76. (view details on MedLine)

  • Maue RA, Burgess RW, Wang B, Wooley CM, Seburn KL, Vanier MT, Rogers MA, Chang CC, Chang TY, Harris BT, Graber DJ, Penatti CA, Porter DM, Szwergold BS, Henderson LP, Totenhagen JW, Trouard TP, Borbon IA, Erickson RP. A novel mouse model of Niemann-Pick type C disease carrying a D1005G-Npc1 mutation comparable to commonly observed human mutations. Hum Mol Genet. 2012 Feb 15;21(4):730-50. (view details on MedLine)

  • Fry, M, Boegle, AK, Maue, RA Differentiated pattern of sodium channel expression in dissociated Purkinje neurons maintained in long-term culture. J Neurochem 2007 May 101(3): 737-748 (view details on MedLine)

  • Paul, CA, Reid PC, Boegle, AK, Karten B, Zhang M, Jiang ZG, Franz D, Lin L, Chang TY, Vance JE, Blanchette-Mackie J, Maue RA Aenovirus expressing an NPC1-GFP fusion gene corrects neuronal and nonneuronal defects associated with Niemann Pick type C disease. J Neurosci Res 2005 Sep 81(5):706-719 (view details on MedLine)

  • Henderson LP, Lin L, Prasad A, Paul CA, Chang TY, Maue RA Embryonic striatal neurons from Niemann Pick type C mice exhibit defects in cholesterol metabolism and neurotrophin responsiveness. J Biol Chem 2000 Jun 30 275(26):20179-87 (view details on MedLine)

  • Murali A, Maue RA, Dolph PJ Reversible symptoms and clearance of mutant prion protein in an inducible model of genetic prion disease in Drosophila Melanogaster. J Neurobiol Dis 2014 (in press)