Sequencing

Extraction Nucleic Acid QC RNA-seq DNA-seq Single Cell Spatial Microarray Sequencing

The GMBSR provides Illumina next-generation sequencing services. Prices include library normalization, pooling, sequencing, and raw data storage.

Use our Cost Estimator Tool for detailed project pricing.

Illumina Sequencing

Variable / see pricing table below
 
  • FASTQ files are stored on Dartmouth's DartFS storage system, accessible to the entire Dartmouth community. Please review our Data Retention Policy for details on storage timelines. Contact Research Computing for a DartFS account.
  • All kits can be run in single-end or paired-end configurations. Sequencing can be purchased as entire flow cells or on a per-read basis for shared runs.
  • NovaSeq runs must be purchased as full flow cells. Most common flow cells are shown below; others available upon request.
NextSeq 2000
Flow Cell Reads Cycles Price
P1 120M 100 $1,181.38
300 $1,415.88
600 $2,005.13
P2 500M 100 $1,507.57
300 $1,799.78
600 $3,533.63
P3 1.3B 100 $2,105.22
300 $3,113.77
P4 2B 100 $2,810.13
300 $4,285.23
NovaSeq 6000
Flow Cell Reads Cycles Price
S2 3.3B 300 $7,921.80
S4 8.5B 300 $11,742.89
Shared Runs (Per-Read Pricing)
Option Reads Price
10M reads 10M $53.33
50M reads 50M $151.81
250M reads 250M $803.50

Shared run prices are based on partial NextSeq 2000 P4 100-cycle run costs. Contact us about partial runs for 300-cycle configurations.

MiniSeq
Kit Reads Cycles Price
Mid Output 8M 300 $957.52
High Output 25M 75 $1,301.80
150 $1,477.52
300 $2,211.53

Prices shown reflect internal Dartmouth rates for FY2027. External users and those with large projects should contact the core directly for pricing information. Actual prices may vary based on batch size.

All services provided by the GMBSR are supported by an NCI Cancer Center Support Grant (5P30CA023108) which MUST be cited in any publications or other materials using data generated in the core.

Single cell sequencing services are provided through the Single Cell Genomics Core and are subsidized by a COBRE grant (P20GM130454). This grant MUST be cited in all publications and materials using single cell data generated in the core.