| Extraction | Nucleic Acid QC | RNA-seq | DNA-seq | Single Cell | Spatial | Microarray | Sequencing | Plasmid/Amplicon-seq | CRISPR Libraries |
The Genomics & Molecular Biology Shared Resource (GMBSR) builds custom CRISPR libraries for targeted screens as one of its core services. We make it easy for investigators to generate small, focused libraries (typically tens to a few hundred targets) for in vitro and in vivo screens, handling everything from guide design through cloning and next-generation sequencing QC. Completed libraries are also maintained by the GMBSR for use by other Dartmouth researchers.
Starting from your gene list, we design the guides, clone the pooled library into a validated vector, and verify guide representation by NGS. The final deliverable is a sequence-verified, NGS-QC’d plasmid library — ready for transduction in your own screen. (Viral packaging is performed by the investigator; the GMBSR does not supply virus.)
Custom library projects begin with a brief, free consultation to confirm the target list, vector choice, and pricing. Contact us at GMBSR@groups.dartmouth.edu to get started.
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STEP 1
Guide Design
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STEP 2
Oligo Synthesis
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STEP 3
Cloning
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STEP 4
Amplification & Maxiprep
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STEP 5
NGS QC & Delivery
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Services & Pricing
Costs below are all-inclusive at internal Dartmouth rates. External users and large projects should contact us for a quote.
Free Consultation & Guide Design: FREE
We start with a no-cost consultation to review your target gene list, select an appropriate vector and reporter, and design guides (5 per target, plus non-targeting and control guides). You receive a project quote before any work begins.
Complete Library Build: $2,130.34
Design and construction of a new custom pooled library from your gene list — oligo-pool synthesis, cloning, high-coverage transformation, and plasmid maxiprep of the ready-to-transduce library.
Library QC: $352.57
Verification of guide representation in a built library by amplicon PCR and sequencing, delivered with a full QC report: total guides recovered, mean/median counts, evenness, and a per-gene PASS/FAIL coverage call.
Re-amplify from Stock: $577.00
For investigators who want to screen with a library the GMBSR already maintains — we re-grow the library from frozen stock and QC it (PCR + sequencing) before delivery.
Vector Repository
The GMBSR maintains the following validated vectors in house. Each entry links to its plasmid map; full vector sequences (GenBank / SnapGene) are available on request — contact us. If you need a different backbone, let us know.
| Vector | Use | Reporter | Variants | Map |
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| pMx-U6-gRNA-PGK-GFP
Empty library-cloning / guide-expression vector
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Library cloning | GFP (PGK) | Ametrine, BFP | ↓ map.pdf |
| lentiCRISPRv2-GFP
All-in-one lentiviral Cas9 + sgRNA
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Cas9 + sgRNA | GFP | BFP, Puro | ↓ map.pdf |
Available Libraries
The following focused CRISPR knockout library designs are maintained by the GMBSR and available to Dartmouth investigators. Each download is the guide/target list for that library. To screen with an existing library, we re-amplify it from stock (see pricing above); contact us to get started.
| Library | Pathway / Focus | Download |
|---|---|---|
| Glycolysis | Central carbon metabolism | ↓ xlsx |
| Pentose Phosphate Pathway | Central carbon metabolism | ↓ xlsx |
| Tricarboxylic Acid (TCA) Cycle | Central carbon metabolism | ↓ xlsx |
| Oxidative Phosphorylation | Mitochondrial metabolism | ↓ xlsx |
| Glutamate Metabolism | Amino-acid metabolism | ↓ xlsx |
| Lipid Metabolism | Lipid metabolism | ↓ xlsx |
| Sphingolipid Metabolism | Lipid metabolism | ↓ xlsx |
| Cell Death | Apoptosis / cell death | ↓ xlsx |
Rates shown reflect internal Dartmouth pricing. External users and large projects should contact us for a quote. Actual cost varies with library size and project scope.
All services provided by the GMBSR are supported by an NCI Cancer Center Support Grant (5P30CA023108) which MUST be cited in any publications or other materials using data generated in the core. The GMBSR’s CRISPR service is additionally supported by a P20 COBRE award and institutional commitments from the Dartmouth Cancer Center and the Geisel School of Medicine at Dartmouth.