DNA-seq: $74.84/sample (based on 30 sample batch)
- Used to prepare sequencing libraries from intact genomic DNA (whole genome sequencing) or sheared DNA (ChIP, CUTNRUN).
- For intact gDNA, samples are fragmented using a Covaris E220 Evolution sonicator.
- Adapters are ligated to fragmented DNA molecules and amplified prior to sequencing.
- Up to 192 samples can be uniquely barcoded for pooled sequencing. Recommended sequencing depth is dependent on application, genome size and desired coverage.
- Please consult with the core to determine your sequencing requirements. A sequencing coverage calculator is also available HERE.
Target Capture (Exome Sequencing): $60.41($100.96)/sample
- For large targeted sequencing studies, including whole exome sequencing, target capture methods are preferred over PCR-based approaches. This is due to the challenge of designing large pools of primers with minimal off-target amplification and coverage bias.
- We use IDT xGen target capture reagents to provide custom target capture as well as whole exome solutions to our users.
- For custom panels, users should use the IDT Design Tool to create and order their panels. IDT's Exome v2 panel is stocked by the core and is included in the exome sequencing price listed above.
Prices shown reflect internal Dartmouth rates for FY2025. External users and those with large projects should contact the core directly for pricing information. Actual prices may vary based on batch size.
All services provided by the GMBSR are supported by an NCI Cancer Center Support Grant (5P30CA023108) which MUST be cited in any publications or other materials using data generated in the core.
Single cell sequencing services are provided through the Single Cell Genomics Core and are subsidized by a COBRE grant (P20GM130454). This grant MUST be cited in all publications and materials using single cell data generated in the core.
Genomics Services
- Nucleic Acid Extraction
- Nucleic Acid QC
- RNA-seq
- DNA-seq
- Single Cell Genomics
- Spatial Genomics
- Microarray
- Sequencing
- Dartmouse