The GMBSR offers a full suite of spatial genomics services in collaboration with the Pathology Shared Resource. All spatial assays include tissue sectioning, staining, imaging, and downstream library preparation where applicable.
Use our Cost Estimator Tool for detailed project pricing.
Visium Tissue Optimization
$1,733.66 / sample
- Necessary step prior to performing the Visium: Fresh Frozen assay.
- In collaboration with Pathology, 7x10um tissue sections are cut onto Visium Tissue Optimization slides and a time course is performed to determine the optimal permeabilization time.
- A separate optimization run should be performed for each tissue type. It is also recommended to separately optimize healthy vs diseased samples.
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Visium Fresh Frozen
$7,004.58 / slide
- Preparation of tissue sections for spatial transcriptomics using the 10x Genomics Visium assay for fresh frozen tissues.
- In collaboration with Pathology, 10um sections are cut and placed on the Visium slide (4 capture areas per slide). H&E staining and imaging (20x) are performed in the Single Cell Genomics Core. Transcripts are detected using a PolyA-based approach.
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Visium FFPE
$9,630.74 / slide
- Performs the 10x Genomics Visium assay on FFPE tissue sections.
- In collaboration with Pathology, 5um FFPE sections are cut onto standard histology slides, followed by H&E staining and 40x whole-slide imaging. Whole transcriptome probes (human or mouse) are hybridized and transferred to the Visium slide using a CytAssist.
- Each Capture Area contains either 5,000 (6.5x6.5mm) or 14,000 (11x11mm) barcoded spots, 55 µm in diameter, providing 1-10 cells per spot resolution.
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Visium HD
$14,730.74 / slide
- Performs the 10x Genomics Visium HD assay on tissue sections.
- In collaboration with Pathology, 5um FFPE sections are cut, H&E stained, and imaged at 40x. Whole transcriptome probes are hybridized and transferred to the Visium slide using CytAssist (2 capture areas per slide).
- Visium HD slides contain ~11 million 2x2 µm barcoded squares without gaps, achieving single cell–scale spatial resolution.
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Xenium Sample Prep
$1,212.44 / slide
- Prepares tissue sections for the 10x Genomics Xenium assay.
- In collaboration with Pathology, tissue sections are cut onto Xenium slides, then probes are hybridized and ligated to their targets. Custom or off-the-shelf probe sets are available from 10x Genomics.
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Xenium
$3,949.10 / slide
- Performs the 10x Genomics Xenium in situ assay on tissue sections.
- Prepared slides are loaded onto the Xenium analyzer, which amplifies and images the targets to build a spatial map of transcripts across the tissue section.
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Prices shown reflect internal Dartmouth rates for FY2026. External users and those with large projects should contact the core directly for pricing information. Actual prices may vary based on batch size.
All services provided by the GMBSR are supported by an NCI Cancer Center Support Grant (5P30CA023108) which MUST be cited in any publications or other materials using data generated in the core.
Single cell sequencing services are provided through the Single Cell Genomics Core and are subsidized by a COBRE grant (P20GM130454). This grant MUST be cited in all publications and materials using single cell data generated in the core.