Single cell genomics experiments are complex and require tight coordination between the researcher and our core. Consultation with core staff is required before projects are accepted and is provided free-of-charge. Services listed include the cost of sample QC using an automated fluorescent cell counter (Nexcellom K2) as well as all downstream library preparation and QC steps.
For all 10x genomics workflows, a "sample" is equivalent to one lane on the 10x instrument. Each lane can capture between 100-20,000 cells, depending on the concentration loaded onto the instrument.
Use our Cost Estimator Tool for detailed project pricing.
Sample Fixation for 10x Genomics Flex
$164.36 / sample
- Samples are provided as cell suspensions or tissues for fixation following the 10x Genomics Flex protocol.
- Cells are stored at -80C in the GMBSR until ready for processing (within 1 year).
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Tissue Processing for 10x Genomics
$268.11 / sample
- Nuclei isolation from fresh/frozen tissue
- Tissue dissociation into single cell suspension (off-the-shelf protocols available for some tissues; those requiring optimization may incur additional charges)
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10x 3'/5' RNA-seq
$2,159.36 / sample
Based on 2 sample batch
- Uses the 10x Genomics workflows for 3' and 5'-End RNA-seq. For standard gene expression, 3' RNA-seq is the standard in the field. 5' RNA-seq is required where TCR and/or BCR (V(D)J) sequencing is performed.
- Up to 96 unique barcodes are available for pooled sequencing.
- Recommended sequencing depth is 30,000 reads per cell.
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10x 3'/5' RNA-seq OCM
$3,431.66 / lane
$857.92/sample · 1 lane of 4 samples
- Uses the 10x Genomics workflows for 3' and 5'-End RNA-seq and incorporates multiplexing.
- Can run up to 4 samples per lane (20,000 cells total)
- Recommended sequencing depth is 30,000 reads per cell.
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10x Library Add-On
$388.51 / library
Based on 2 sample batch
- Additional modalities can be applied to 10x RNA-seq samples to generate multi-omics datasets at single cell resolution. These include TCR/BCR sequencing, proteomic analyses (CITE-seq), sample multiplexing (MULTI-seq or Total-seq) and other custom assays.
- For each additional modality, a Library Add-On is required per sample.
- Additional sequencing is also required, typically 5,000 reads per cell per library type.
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10x Gene Expression Flex
Variable / lane
A.K.A. Fixed RNA Profiling
- Enables single cell RNA-seq analysis of Human or Mouse cells or tissues that have been fixed following 10x Demonstrated Protocols. Samples can be stored at -80C for up to 6 months.
- Pooling configurations: 1 Barcode: $3,289.07/lane · 4 Barcodes: $5,472.79/lane · 16 Barcodes: $11,140.23/lane
- Recommended sequencing depth is 20,000 reads/cell.
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10x ATAC-seq
$2,502.91 / sample
Based on 2 sample batch
- The 10x ATAC-seq workflow interrogates regions of open chromatin at single cell resolution.
- Users provide a viable single cell suspension; the core performs all downstream processing including nuclei isolation/permeabilization, DNA transposition, cell capture and library preparation.
- Up to 96 unique barcodes are available for pooled sequencing.
- Recommended sequencing depth is 25,000 reads per cell.
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10x Multiome (RNA+ATAC)
$4,141.60 / sample
Based on 2 sample batch
- Generates paired RNA-seq and ATAC-seq data from the same nuclei in a given sample.
- Specific requirements exist for preparing nuclei suspensions; this is included in the cost of the service. Users providing their own nuclei should consult the GMBSR and 10x Genomics website.
- Sequencing requirements are 25,000 reads per cell each for RNA and ATAC datasets (50,000 reads/cell total).
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10x Instrument Training
FREE
- Core staff are available 8am-5pm, Monday-Friday to perform 10x experiments. Users needing access outside these hours can be trained to operate the instrument independently.
- Training takes ~2 hours and includes discussion of the 10x workflow principles as well as hands-on time with the instrument.
- Trained users will be provided access to the lab as well as the instrument reservation calendar via RaDar.
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Parse Bioscience scRNA-seq
Contact for Pricing
- Combinatorial barcoding platform for single cell RNA-seq that does not require a microfluidics instrument.
- Scalable to hundreds of thousands or millions of cells per experiment.
- Compatible with fixed and frozen samples, enabling flexible experimental timelines.
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Bioskryb Combined scDNA/RNA-seq
Contact for Pricing
- Enables simultaneous analysis of genomic DNA and RNA from the same single cell.
- Ideal for studying somatic mutations, copy number variations, and gene expression in the same cell population.
- Contact the GMBSR for project consultation and experimental design.
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Prices shown reflect internal Dartmouth rates for FY2026. External users and those with large projects should contact the core directly for pricing information. Actual prices may vary based on batch size.
All services provided by the GMBSR are supported by an NCI Cancer Center Support Grant (5P30CA023108) which MUST be cited in any publications or other materials using data generated in the core.
Single cell sequencing services are provided through the Single Cell Genomics Core and are subsidized by a COBRE grant (P20GM130454). This grant MUST be cited in all publications and materials using single cell data generated in the core.