Instruments

Sequencing/NGS


silver and purple machine

Illumina NextSeq2000

  • Capable of producing 100M-1.2B reads/run
  • Applications include:
    • 40 RNA-seq libraries
    • 40 Human exomes (200x)
    • 4 10x scRNA-seq samples (10K cells)
    • High throughput 16s, ITS and TCR/BCR-seq with 600 cycle kits
  • Common run configurations:
    • 1x50, 1x100, 2x50, 2x150 and 2x300

black and white machine

Illumina MiniSeq

  • 7M-30M reads/run
  • Applications include:
    • Targeted sequencing: 700 amplicons in 30 samples
    • 10 miRNA-seq samples
    • 25-50 small whole genomes
  • Common run configurations:
    • 1x75, 2x75 or 2x150bp

Eppendorf EpMotion 5075t

  • Automated liquid handling for NGS workflows
  • Accesses tubes and 96/384 well plate formats
  • 14 deck positions available
  • 1 thermal block and 1 thermomixer on deck

 

Single Cell/Spatial 


black and white square machine

10x Genomics Chromium X

  • High throughput single cell applications
    • 5'/3' mRNA-seq
    • TCR/BCR-seq
    • ATAC-seq
    • CITE-seq (Surface Protein)
    • Multiome (RNA+ATAC)
    • scRNA-seq on fixed/FFPE samples

black square machine

10x Genomics CytAssist

  • Flexible workflow for spatial transcriptomics on FFPE samples using the Visium technology
  • Tissues are sectioned onto standard histology slides, H&E stained, imaged at 40x and transfered to the Visium slide via the CytAssist
  • Supports surface protein detection using 10x validated reagents

10X Genomics Xenium Analyzer

  • Platform for in situ analysis
  • Characterize 100s–1,000s of genes in cells and tissues with ultra precise single cell spatial imaging
  • Tissue is sectioned onto Xenium slides, then probes are hybridized and ligated to their targets.  The amplified targets are analyzed to build a spatial map of the transcripts across the tissue section

white small machine

Cytiva VIA Extractor

  • Automated processing of tissues into single cell suspensions
  • Full control over temperature, homogenization intensity and run time
  • Flexible system for processing 1-3 samples in parallel in 1-5ml of dissociation mix

rectangular white box

Nexcelom Cellometer K2 Automated Cell Counter

  • Fluorescence-based staining and counting of cells/nuclei using AO/PI dye
  • Cell size measurements
  • Tunable counting parameters based on cell size, fluorescence intensity and cell aggregate declustering
  • PDF results report

small white square machine

Curiox Laminar Wash Mini

  • Centrifuge-free, laminar flow based washing system
  • Cells undergo a gentle wash without added stress,  enabling increased cell retention and better data quality
  • Great for CITEseq and sample multiplexing

 

QC


white machine connected to computer

Agilent Fragment Analyzer

  • Quality assessment of DNA and RNA
    • gDNA
    • NGS libraries
    • smRNA
    • Total RNA
  • 96 well plate format for higher throughput

large white machine

Agilent TapeStation

  • Quick quality assessment of DNA and RNA
    • gDNA
    • NGS libraries
    • smRNA
    • Total RNA

small rectangualr machine with touch screen

Invitrogen Qubit Flex

  • A benchtop fluorometer that provides highly accurate quantification of DNA and RNA
  • Florescence-based measurements are more sensitive than UV absorbance, especially for low concentration samples
  • Measure 8 samples at a time