Before submitting samples, place an order through the RaDar system. Please complete any required order forms for the service and provide an active account number.
Place your samples in a box labeled with your name, lab, sample type and RaDar number. Label your tubes to match the submission form (we prefer short labels!)
You can drop off the samples at the GMBSR, located at Rubin 670 in the Dartmouth Cancer Center, Lebanon, NH between the hours of 8am and 5pm. You can reach us at GMBSR@groups.dartmouth.edu or by phone at (603) 646-5502 if you have trouble accessing the lab.
Sample Requirements
DNA/RNA extraction
Cell samples: snap‑freeze on dry ice or homogenize in Qiagen RLT buffer and store at ‑80 °C prior to submission
Tissue samples: snap‑freeze or store in RNAlater at ‑80 °C until submission
Input: 10,000-10,000,000 for cells, 1-10mg for tissue
RNAseq
Note: All RNA samples should be QC’d by Qubit and TapeStation before beginning. If you need us to do the QC, please provide a few extra ul of sample. If you have quantified the sample with Nanodrop, please provide extra sample because the Nanodrop often overestimates the concentration.
Ribodepletion RNA-seq:100 ng–1 µg of total RNA (RIN >8 recommended)
3′ End-Counting RNA-seq: around 100 ng of total RNA (RIN >8 recommended)
miRNA/smRNA-seq:100 ng–1 µg total RNA.
Low Input RNA-seq: 250 pg–10 ng total RNA.
DNAseq
Note: All DNA samples should be QC’d by Qubit before beginning. If you need us to do the QC, please provide a few extra ul of sample. If you have quantified the sample with Nanodrop, please provide extra sample because the Nanodrop often overestimates the concentration.
Standard DNA-seq (whole genome or sheared DNA for ChIP-seq/CUT&RUN): a minimum of 1–10 ng of DNA is typically sufficient, with higher input (>100 ng) preferred
Target Capture / Exome Sequencing: at least 100–200 ng of genomic DNA
Single Cell
Each 10x lane can capture 100–20,000 cells. Please provide at least 1.5X the number of cells you want to capture (30,000 if you want to capture 20,000).
For multiome, please provide at least 100,000 cells per sample.
Note: Cell viability should ideally be >85%, and cell concentrations should be verified with a fluorescent cell counter. The sorter overestimates the cell count.
Arrays
Standard: 250 ng of DNA
FFPE: 500 ng-1 µg of DNA
Note: Methylation arrays consist of 8 (human) or 12 (mouse) samples each and must be run in full