Edit Entry | Edit CV

George A. O'Toole Jr, PhD

Professor of Microbiology and Immunology

Microbiology and Immunology

University of Wisconsin - Madison, Ph.D., 1994
Cornell University, B.S., 1988

After postdoctoral work at the University of Wisconsin-Madison and Harvard Medical School, Dr. O'Toole joined the faculty of the Department of Microbiology at Dartmouth Medical School in 1999.

Molecular and Cellular Biology Graduate Programs

O'Toole Lab
Microbiology and Molecular Pathogenesis Program
Dept of Microbiology & Immunology
Molecular Cellular Biology Grad Program

Academic Analytics
View Profile

Contact Information

Geisel School of Medicine
Remsen Building, Rm 202 - HB 7550
Hanover NH 03755

Office: 202 Remsen
Phone: 603-650-1248
Email: georgeo@Dartmouth.Edu

Professional Interests

The main focus of the O'Toole laboratory is the study of complex surface-attached bacterial communities known as biofilms. Biofilms can form on a wide variety of surfaces including catheter lines, surgical implants, contact lenses, the lungs of patients with cystic fibrosis, industrial and drinking water pipelines, and on the surfaces of plant roots. In most natural, clinical, and industrial settings bacteria live predominantly in biofilms and not as planktonic (free-swimming) cells such as those typically studied in the laboratory. Bacteria growing in biofilm communities are of great interest to the medical community, because these bacteria become highly resistant to antibiotics by an as yet unknown mechanism. Although much has been learned about the types of microbes that can form biofilms, the morphology of these communities, and their chemical/physical properties, until recently little was known about the molecular genetic basis of biofilm formation or antibiotic resistance.

Studies in the O'Toole lab focus on:
>Polymicrobial infections and antibiotic tolerance in cystic fibrosis.
>The role of gut microbiota in airway disease in infants with cystic fibrosis.
>The signal transduction pathways regulating biofilm formation and surface sensing.
>The role of the intracellular signaling molecule c-di-GMP in controlling biofilm formation by Pseudomonads.

Selected Publications


Multiple pathways impact the swarming motility of Pseudomonas fluorescens Pf0-1.
Pastora AB, Rzasa KM, O'Toole GA
Microbiol Spectr. 2024 Apr 30;:e0016624. doi: 10.1128/spectrum.00166-24. Epub 2024 Apr 30.
PMID: 38687073

It's time to write a minireview.
O'Toole GA
J Bacteriol. 2024 Apr 16;:e0011324. doi: 10.1128/jb.00113-24. Epub 2024 Apr 16.
PMID: 38624220

Local control: a hub-based model for the c-di-GMP network.
Vasenina A, Fu Y, O'Toole GA, Mucha PJ
mSphere. 2024 Apr 9;:e0017824. doi: 10.1128/msphere.00178-24. Epub 2024 Apr 9.
PMID: 38591888

We have a community problem.
O'Toole GA
J Bacteriol. 2024 Apr 18;206(4):e0007324. doi: 10.1128/jb.00073-24. Epub 2024 Mar 26.
PMID: 38529952

Reconstitution of a biofilm adhesin system from a sulfate-reducing bacterium in Pseudomonas fluorescens.
Karbelkar AA, Font M, Smith TJ, Sondermann H, O'Toole GA
Proc Natl Acad Sci U S A. 2024 Mar 26;121(13):e2320410121. doi: 10.1073/pnas.2320410121. Epub 2024 Mar 18.
PMID: 38498718

How P. aeruginosa cells with diverse stator composition collectively swarm.
de Anda J, Kuchma SL, Webster SS, Boromand A, Lewis KA, Lee CK, Contreras M, Medeiros Pereira VF, Schmidt W, Hogan DA, O'Hern CS, O'Toole GA, Wong GCL
mBio. 2024 Apr 10;15(4):e0332223. doi: 10.1128/mbio.03322-23. Epub 2024 Mar 1.
PMID: 38426789

Quantifying forms and functions of intestinal bile acid pools in mice.
Sudo K, Delmas-Eliason A, Soucy S, Barrack KE, Liu J, Balasubramanian A, Shu CJ, James M, Hegner CL, Dionne HD, Rodriguez-Palacios A, Krause H, O'Toole GA, Karpen SJ, Dawson PA, Schultz D, Sundrud MS
bioRxiv. 2024 Feb 18; pii: 2024.02.16.580658. doi: 10.1101/2024.02.16.580658. Epub 2024 Feb 18.
PMID: 38405928

Multiple Pathways Impact Swarming Motility of Pseudomonas fluorescens Pf0-1.
Pastora AB, Rzasa KM, O'Toole GA
bioRxiv. 2024 Jan 18; pii: 2024.01.17.576057. doi: 10.1101/2024.01.17.576057. Epub 2024 Jan 18.
PMID: 38293239

Binding of GTP to BifA is required for the production of Pel-dependent biofilms in Pseudomonas aeruginosa.
Van Loon JC, Whitfield GB, Wong N, O'Neal L, Henrickson A, Demeler B, O'Toole GA, Parsek MR, Howell PL
J Bacteriol. 2024 Feb 22;206(2):e0033123. doi: 10.1128/jb.00331-23. Epub 2024 Jan 10.
PMID: 38197635

Intestinal Bacteroides modulates inflammation, systemic cytokines, and microbial ecology via propionate in a mouse model of cystic fibrosis.
Price CE, Valls RA, Ramsey AR, Loeven NA, Jones JT, Barrack KE, Schwartzman JD, Royce DB, Cramer RA, Madan JC, Ross BD, Bliska J, O'Toole GA
mBio. 2024 Feb 14;15(2):e0314423. doi: 10.1128/mbio.03144-23. Epub 2024 Jan 5.
PMID: 38179971

View more publications on PubMed