Spatial Genomics

Visium Tissue Optimization: $908/sample

  • This service is a necessary step prior to performing the Visium: Fresh Frozen assay.
  • In collaboration with the Pathology Shared Resource, 7x10um tissue sections are cut onto Visium Tissue Optimization slides and a time course is performed to determine the optimal tissue permeabilization time for the assay.
  • A separate tissue optimization run should be performed for each tissue type to be tested. It is also recommended to separately optimize healthy vs diseased samples, if significant differences in tissue architecture or properties exist.

Visium Fresh Frozen: $5857/sample

  • This service is for the preparation of tissue sections for spatial transriptomics using the 10x Genomics Visium assay for fresh frozen tissues.
  • In collaboration with the Pathology Shared Resource (PSR), 10um sections are cut and placed directly on the Visium slide. H&E staining and imaging (20x) are performed in the Single Cell Genomics Core. Transcripts are detected using a PolyA-based approach, making the assay applicable to a wide range of species including human, mouse, rat, zebrafish and more.

Visium FFPE: $6838/sample

  • This service is to perform the 10x Genomics Visium assay on FFPE tissue sections.
  • In collaboration with the Pathology Shared Resource, 5um FFPE sections are cut onto standard histology slides, followed by clinical-grade H&E staining and 40x whole-slide imaging. probes targeting the whole transcriptome are hybridized to the tissue section and transferred to the Visium slide using a 10x Genomics CytAssist to preserve the spatial distribution of the probes.

Prices shown reflect internal Dartmouth rates for FY2024. External users and those with large projects should contact the core directly for pricing information. Actual prices may vary based on batch size.

All services provided by the GMBSR are supported by an NCI Cancer Center Support Grant (5P30CA023108) which MUST be cited in any publications or other materials using data generated in the core.

Single cell sequencing services are provided through the Single Cell Genomics Core and are subsidized by a COBRE grant (P20GM130454). This grant MUST be cited in all publications and materials using single cell data generated in the core.

Genomics Services