Sequencing/NGS
Illumina NextSeq2000
- Capable of producing 100M-1.2B reads/run
- Applications include:
- 40 RNA-seq libraries
- 40 Human exomes (200x)
- 4 10x scRNA-seq samples (10K cells)
- High throughput 16s, ITS and TCR/BCR-seq with 600 cycle kits
- Common run configurations:
- 1x50, 1x100, 2x50, 2x150 and 2x300
Illumina MiniSeq
- 7M-30M reads/run
- Applications include:
- Targeted sequencing: 700 amplicons in 30 samples
- 10 miRNA-seq samples
- 25-50 small whole genomes
- Common run configurations:
- 1x75, 2x75 or 2x150bp
Eppendorf EpMotion 5075t
- Automated liquid handling for NGS workflows
- Accesses tubes and 96/384 well plate formats
- 14 deck positions available
- 1 thermal block and 1 thermomixer on deck
Single Cell/Spatial
10x Genomics Chromium X
- High throughput single cell applications
- 5'/3' mRNA-seq
- TCR/BCR-seq
- ATAC-seq
- CITE-seq (Surface Protein)
- Multiome (RNA+ATAC)
- scRNA-seq on fixed/FFPE samples
10x Genomics CytAssist
- Flexible workflow for spatial transcriptomics on FFPE samples using the Visium technology
- Tissues are sectioned onto standard histology slides, H&E stained, imaged at 40x and transfered to the Visium slide via the CytAssist
- Supports surface protein detection using 10x validated reagents
10X Genomics Xenium Analyzer
- Platform for in situ analysis
- Characterize 100s–1,000s of genes in cells and tissues with ultra precise single cell spatial imaging
- Tissue is sectioned onto Xenium slides, then probes are hybridized and ligated to their targets. The amplified targets are analyzed to build a spatial map of the transcripts across the tissue section
Cytiva VIA Extractor
- Automated processing of tissues into single cell suspensions
- Full control over temperature, homogenization intensity and run time
- Flexible system for processing 1-3 samples in parallel in 1-5ml of dissociation mix
Nexcelom Cellometer K2 Automated Cell Counter
- Fluorescence-based staining and counting of cells/nuclei using AO/PI dye
- Cell size measurements
- Tunable counting parameters based on cell size, fluorescence intensity and cell aggregate declustering
- PDF results report
Curiox Laminar Wash Mini
- Centrifuge-free, laminar flow based washing system
- Cells undergo a gentle wash without added stress, enabling increased cell retention and better data quality
- Great for CITEseq and sample multiplexing
QC
Agilent Fragment Analyzer
- Quality assessment of DNA and RNA
- gDNA
- NGS libraries
- smRNA
- Total RNA
- 96 well plate format for higher throughput
Agilent TapeStation
- Quick quality assessment of DNA and RNA
- gDNA
- NGS libraries
- smRNA
- Total RNA
Invitrogen Qubit Flex
- A benchtop fluorometer that provides highly accurate quantification of DNA and RNA
- Florescence-based measurements are more sensitive than UV absorbance, especially for low concentration samples
- Measure 8 samples at a time