Protocols

Calcium flux (flow cytometry; Indo-1)

CFSE cell labeling

DP Thymocyte Enrichment

pAKT intracellular stain

PIP pulldowns

RuCl Competent Bacteria

SDS-PAGE Western blot analysis

Thymocyte Stimulation for Immunoprecipitation

Transforming chemically competent bacteria

Transwell Migration Assay