June 12-16, 2023 – RNA-seq analysis workshop 2023

The DAC aims to facilitate advanced bioinformatic, computational, and statistical analysis of complex genomics data for the Dartmouth research community.

Hosted by Data Analytics Core, DataAnalyticsCore@groups.dartmouth.edu

Location Zoom link to be provided

Monday, June 12, 2023, 12-5pm

Tuesday, June 13, 2023, 12-5pm

Thursday, June 15, 2023, 12-5pm

Friday, June 16, 2023, 12-5pm

This workshop series introduces bulk RNA-seq data analysis, with a particular emphasis on differential expression analysis.


Part I of this workshop, days 1 and 2, will discuss data generation, QC, and data reduction:

  • Develop a working understanding of the analytical workflow for a modern RNA-seq experiment
  • Build a working knowledge of sample preparation considerations for RNA-seq experiments
  • Learn how to process raw NGS data in FASTQ format to generate a gene expression matrix
  • Learn how to perform a detailed quality control analysis of RNA-seq data

Part II of this workshop, days 3 and 4, will cover differential expression (DE) analysis.

  • Develop a working understanding of fundamental bioinformatics and statistical concepts for a typical bulk RNA-seq DE analysis
  • Learn how to leverage the R/Bioconductor framework to perform DE analysis
  • Learn how to use unsupervised data analysis methods (e.g. principal components analysis) to explore RNA-seq datasets
  • Perform a complete DE analysis on a real RNA-seq dataset

Registration Fees are as follows:

CQB Labs : $0
CQB Mentor Labs : $120
NH-INBRE affiliates : $120
Dartmouth College, not CQB affiliated : $240
External Academic Attendees: $360
Non-Academic Attendees : $600