{"id":26,"date":"2024-10-16T22:06:26","date_gmt":"2024-10-16T22:06:26","guid":{"rendered":"https:\/\/geiselmed2.dartmouth.edu\/ms\/?page_id=26"},"modified":"2025-01-22T19:20:55","modified_gmt":"2025-01-22T19:20:55","slug":"instrumentation","status":"publish","type":"page","link":"https:\/\/geiselmed.dartmouth.edu\/msp\/instrumentation\/","title":{"rendered":"Instrumentation"},"content":{"rendered":"
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Mass Spectrometers<\/h2>\n
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Orbitrap Fusion<\/h3>\n

The Thermo Scientific Orbitrap Fusion Tribrid\u2122 mass spectrometer is equipped with three mass analyzers that permit complex MSn<\/sup>\u00a0experimental design: a quadrupole, an Orbitrap and a linear ion trap. CID, HCD, and ETD fragmentation modes, scan speeds up to 20 Hz, resolution up to 500,000 (FWHM) at m\/z 200 in the Orbitrap.<\/p>\n<\/div><\/div>

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Orbitrap Fusion Lumos<\/h3>\n

The Thermo Scientific Orbitrap Fusion Lumos Tribrid\u2122 Mass Spectrometer provides key improvements to Fusion that enhance analytical performance, including a brighter ion source, a segmented quadrupole with higher selectivity, and Advanced Vacuum Technology for improved transmission.<\/p>\n<\/div><\/div>

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Q Exactive Plus<\/h3>\n

The Thermo Scientific Q Exactive\u2122 Plus\u00a0benchtop LC-MS offers high performance quadrupole precursor selection followed by high-resolution,\u00a0accurate-mass (HR\/AM) full scan Orbitrap\u00a0detection. Sub-ppm mass accuracy, scan speeds up to 12 Hz,\u00a0mass resolution up to 140,000 (FWHM) at\u00a0m\/z 200.<\/p>\n<\/div><\/div>

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HPLCs<\/h2>\n
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ACQUITY Arc<\/h3>\n

The Waters ACQUITY Arc is used for uHPLC pre-fractionation of complex peptide mixtures. Common applications include off-line fractionation of TMT-labeled peptides from whole proteome or phosphoproteome preparations and fractionation of unlabeled peptides for spectral library generation.<\/p>\n<\/div><\/div>

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Easy-nLC 1000<\/h3>\n

The Thermo Scientific Easy nLC\u2122 1000 is a nano-flow UHPLC with a pressure rating of 1000 bar and is used for on-line separation with the Q Exactive\u2122 Plus.<\/p>\n<\/div><\/div>

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Easy-nLC 1200<\/h3>\n

The Thermo Scientific Easy nLC\u2122 1200 is a nano-flow UHPLC with a pressure rating of 1200 bar and is used for on-line separation with the Orbitrap Fusion Tribrid\u2122.<\/p>\n<\/div><\/div>

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Vanquish Neo<\/h3>\n

The Thermo Scientific Vanquish Neo UHPLC system delivers reproducible nano-flow LC-MS with a pressure rating of 1500 bar. The Vanquish is used for on-line separation with the Orbitrap Fusion Lumos Tribrid\u2122.<\/p>\n<\/div><\/div>

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Mass Spectrometers HPLCs<\/p>\n","protected":false},"author":9,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-26","page","type-page","status-publish","hentry","author-9"],"_links":{"self":[{"href":"https:\/\/geiselmed.dartmouth.edu\/msp\/wp-json\/wp\/v2\/pages\/26","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/geiselmed.dartmouth.edu\/msp\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/geiselmed.dartmouth.edu\/msp\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/msp\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/msp\/wp-json\/wp\/v2\/comments?post=26"}],"version-history":[{"count":3,"href":"https:\/\/geiselmed.dartmouth.edu\/msp\/wp-json\/wp\/v2\/pages\/26\/revisions"}],"predecessor-version":[{"id":336,"href":"https:\/\/geiselmed.dartmouth.edu\/msp\/wp-json\/wp\/v2\/pages\/26\/revisions\/336"}],"wp:attachment":[{"href":"https:\/\/geiselmed.dartmouth.edu\/msp\/wp-json\/wp\/v2\/media?parent=26"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}