{"id":974,"date":"2026-08-05T19:16:39","date_gmt":"2026-08-05T19:16:39","guid":{"rendered":"https:\/\/geiselmed.dartmouth.edu\/gsr\/?page_id=974"},"modified":"2026-08-05T19:19:35","modified_gmt":"2026-08-05T19:19:35","slug":"samplesubmission","status":"publish","type":"page","link":"https:\/\/geiselmed.dartmouth.edu\/gsr\/samplesubmission\/","title":{"rendered":"Submitting Samples to the GMBSR"},"content":{"rendered":"<table style=\"border-collapse: separate;border-spacing: 20px;margin: 0 -20px\">\n<tbody>\n<tr>\n<td style=\"vertical-align: top;border: 1px solid #ddd;border-left: 4px solid #00693e;border-radius: 8px;padding: 24px;background: #fff\" width=\"50%\">\n<h3 style=\"margin: 0 0 4px 0;font-size: 18px;color: #333\">Sample Submission Guidelines<\/h3>\n<ul style=\"margin: 0;padding-left: 20px\">\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Before submitting samples, place an order through the <a href=\"http:\/\/radar.dartmouth.edu\">RaDar<\/a> system. Please complete any required order forms for the service and provide an active account number.<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Place your samples in a box labeled with your name, lab, sample type and RaDar number. Label your tubes to match the submission form (we prefer short labels!)<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">You can drop off the samples at the GMBSR, located at Rubin 670 in the Dartmouth Cancer Center, Lebanon, NH between the hours of 8am and 5pm.\u00a0 You can reach us at <a href=\"mailto:GMBSR@groups.dartmouth.edu\">GMBSR@groups.dartmouth.edu<\/a> or by phone at (603) 646-5502 if you have trouble accessing the lab.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 30pt\"><strong>Sample Requirements<\/strong><\/span><\/p>\n<table style=\"border-collapse: separate;border-spacing: 20px;margin: 0 -20px\">\n<tbody>\n<tr>\n<td style=\"vertical-align: top;border: 1px solid #ddd;border-left: 4px solid #00693e;border-radius: 8px;padding: 24px;background: #fff\" width=\"50%\">\n<h3 style=\"margin: 0 0 4px 0;font-size: 18px;color: #333\">DNA\/RNA extraction<\/h3>\n<ul>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Cell samples: snap\u2011freeze on dry ice <em>or<\/em> homogenize in Qiagen RLT buffer and store at \u201180\u202f\u00b0C prior to submission<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Tissue samples: snap\u2011freeze <em>or<\/em> store in RNAlater at \u201180\u202f\u00b0C until submission<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Input: 10,000-10,000,000 for cells, 1-10mg for tissue<\/li>\n<\/ul>\n<\/td>\n<td style=\"vertical-align: top;border: 1px solid #ddd;border-left: 4px solid #00693e;border-radius: 8px;padding: 24px;background: #fff\" width=\"50%\">\n<h3 style=\"margin: 0 0 4px 0;font-size: 18px;color: #333\">RNAseq<\/h3>\n<ul>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\"><em>Note:<\/em> All RNA samples should be QC\u2019d by Qubit and TapeStation before beginning. If you need us to do the QC, please provide a few extra ul of sample.\u00a0 If you have quantified the sample with Nanodrop, please provide extra sample because the Nanodrop often overestimates the concentration.<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Ribodepletion RNA-seq:100 ng\u20131 \u00b5g of total RNA (RIN &gt;8 recommended)<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">3\u2032 End-Counting RNA-seq: around 100 ng of total RNA (RIN &gt;8 recommended)<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">miRNA\/smRNA-seq:100 ng\u20131 \u00b5g total RNA.<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Low Input RNA-seq: 250 pg\u201310 ng total RNA.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"vertical-align: top;border: 1px solid #ddd;border-left: 4px solid #00693e;border-radius: 8px;padding: 24px;background: #fff\" width=\"50%\">\n<h3 style=\"margin: 0 0 4px 0;font-size: 18px;color: #333\">DNAseq<\/h3>\n<ul>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\"><em>Note:<\/em> All DNA samples should be QC\u2019d by Qubit before beginning. If you need us to do the QC, please provide a few extra ul of sample.\u00a0 If you have quantified the sample with Nanodrop, please provide extra sample because the Nanodrop often overestimates the concentration.<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Standard DNA-seq (whole genome or sheared DNA for ChIP-seq\/CUT&amp;RUN): a minimum of 1\u201310 ng of DNA is typically sufficient, with higher input (&gt;100 ng) preferred<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Target Capture \/ Exome Sequencing: at least 100\u2013200 ng of genomic DNA<\/li>\n<\/ul>\n<\/td>\n<td style=\"vertical-align: top;border: 1px solid #ddd;border-left: 4px solid #00693e;border-radius: 8px;padding: 24px;background: #fff\" width=\"50%\">\n<h3 style=\"margin: 0 0 4px 0;font-size: 18px;color: #333\">Single Cell<\/h3>\n<ul>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Each 10x lane can capture 100\u201320,000 cells. Please provide at least 1.5X the number of cells you want to capture (30,000 if you want to capture 20,000).<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">For multiome, please provide at least 100,000 cells per sample.<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\"><em>Note:<\/em> Cell viability should ideally be &gt;85%, and cell concentrations should be verified with a fluorescent cell counter. The sorter overestimates the cell count.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"vertical-align: top;border: 1px solid #ddd;border-left: 4px solid #00693e;border-radius: 8px;padding: 24px;background: #fff\" width=\"50%\">\n<h3 style=\"margin: 0 0 4px 0;font-size: 18px;color: #333\">Arrays<\/h3>\n<ul>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Standard: 250 ng of DNA<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">FFPE: 500 ng-1 \u00b5g of DNA<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\"><em>Note:<\/em> Methylation arrays consist of 8 (human) or 12 (mouse) samples each and must be run in full<\/li>\n<\/ul>\n<\/td>\n<td style=\"vertical-align: top;border: 1px solid #ddd;border-left: 4px solid #00693e;border-radius: 8px;padding: 24px;background: #fff\" width=\"50%\">\n<h3 style=\"margin: 0 0 4px 0;font-size: 18px;color: #333\">Nanopore<\/h3>\n<ul>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">500 ng-1 \u00b5g of high molecular weight DNA<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"vertical-align: top;border: 1px solid #ddd;border-left: 4px solid #00693e;border-radius: 8px;padding: 24px;background: #fff\" width=\"50%\">\n<h3 style=\"margin: 0 0 4px 0;font-size: 18px;color: #333\">Plasmid\/Amplicon Sequencing<\/h3>\n<ul>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Plasmids: At least 20 ng\/\u00b5L in 20 \u00b5L volume<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">Amplicons: 100 ng in 20 \u00b5L (5 ng\/\u00b5L)<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Sample Submission Guidelines Before submitting samples, place an order through the RaDar system. Please complete any required order forms for the service and provide an active account number. Place your samples in a box labeled with your name, lab, sample type and RaDar number. Label your tubes to match the [\u2026] <\/p>\n<div class=\"clear\"><\/div>\n<p><a class=\"more_link clearfix\" href=\"https:\/\/geiselmed.dartmouth.edu\/gsr\/samplesubmission\/\" rel=\"nofollow\">Read More<\/a><\/p>\n","protected":false},"author":145,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-974","page","type-page","status-publish","hentry","author-145"],"jetpack_shortlink":"https:\/\/wp.me\/PbaUZP-fI","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/pages\/974","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/users\/145"}],"replies":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/comments?post=974"}],"version-history":[{"count":20,"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/pages\/974\/revisions"}],"predecessor-version":[{"id":1017,"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/pages\/974\/revisions\/1017"}],"wp:attachment":[{"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/media?parent=974"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}