{"id":629,"date":"2024-05-20T18:26:30","date_gmt":"2024-05-20T18:26:30","guid":{"rendered":"https:\/\/geiselmed.dartmouth.edu\/gsr\/?page_id=629"},"modified":"2026-04-28T15:30:48","modified_gmt":"2026-04-28T15:30:48","slug":"sequencing","status":"publish","type":"page","link":"https:\/\/geiselmed.dartmouth.edu\/gsr\/sequencing\/","title":{"rendered":"Sequencing"},"content":{"rendered":"<table style=\"border-collapse: separate;border-spacing: 6px 6px;margin: 0 0 20px -6px\">\n<tbody>\n<tr>\n<td style=\"padding: 7px 14px;border-radius: 999px;background: #f5f5f5;border: 1px solid #ddd;font-size: 13px;text-align: center;white-space: nowrap\"><a href=\"https:\/\/geiselmed.dartmouth.edu\/gsr\/nucleicacidextraction\/\" style=\"color: #555;text-decoration: none\">Extraction<\/a><\/td>\n<td style=\"padding: 7px 14px;border-radius: 999px;background: #f5f5f5;border: 1px solid #ddd;font-size: 13px;text-align: center;white-space: nowrap\"><a href=\"https:\/\/geiselmed.dartmouth.edu\/gsr\/nucleic-acid-qc\/\" style=\"color: #555;text-decoration: none\">Nucleic Acid QC<\/a><\/td>\n<td style=\"padding: 7px 14px;border-radius: 999px;background: #f5f5f5;border: 1px solid #ddd;font-size: 13px;text-align: center;white-space: nowrap\"><a href=\"https:\/\/geiselmed.dartmouth.edu\/gsr\/rna-seq\/\" style=\"color: #555;text-decoration: none\">RNA-seq<\/a><\/td>\n<td style=\"padding: 7px 14px;border-radius: 999px;background: #f5f5f5;border: 1px solid #ddd;font-size: 13px;text-align: center;white-space: nowrap\"><a href=\"https:\/\/geiselmed.dartmouth.edu\/gsr\/dna-seq\/\" style=\"color: #555;text-decoration: none\">DNA-seq<\/a><\/td>\n<td style=\"padding: 7px 14px;border-radius: 999px;background: #f5f5f5;border: 1px solid #ddd;font-size: 13px;text-align: center;white-space: nowrap\"><a href=\"https:\/\/geiselmed.dartmouth.edu\/gsr\/single-cell-genomics\/\" style=\"color: #555;text-decoration: none\">Single Cell<\/a><\/td>\n<td style=\"padding: 7px 14px;border-radius: 999px;background: #f5f5f5;border: 1px solid #ddd;font-size: 13px;text-align: center;white-space: nowrap\"><a href=\"https:\/\/geiselmed.dartmouth.edu\/gsr\/spatial-genomics\/\" style=\"color: #555;text-decoration: none\">Spatial<\/a><\/td>\n<td style=\"padding: 7px 14px;border-radius: 999px;background: #f5f5f5;border: 1px solid #ddd;font-size: 13px;text-align: center;white-space: nowrap\"><a href=\"https:\/\/geiselmed.dartmouth.edu\/gsr\/microarray\/\" style=\"color: #555;text-decoration: none\">Microarray<\/a><\/td>\n<td style=\"padding: 7px 14px;border-radius: 999px;background: #00693e;color: #fff;font-size: 13px;font-weight: 500;text-align: center;white-space: nowrap\">Sequencing<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>The GMBSR provides Illumina next-generation sequencing services. Prices include library normalization, pooling, sequencing, and raw data storage.<\/p>\n<p>Use our <a href=\"https:\/\/omics.dartmouth.edu\/genomics-and-molecular-biology\/genomics-cost-calculator\/\">Cost Estimator Tool<\/a> for detailed project pricing.<\/p>\n<table style=\"border-collapse: separate;border-spacing: 20px;margin: 0 -20px\">\n<tbody>\n<tr>\n<td width=\"50%\" style=\"vertical-align: top;border: 1px solid #ddd;border-left: 4px solid #00693e;border-radius: 8px;padding: 24px;background: #fff\">\n<h3 style=\"margin: 0 0 4px 0;font-size: 18px;color: #333\">Illumina Sequencing<\/h3>\n<div style=\"font-size: 22px;font-weight: 600;color: #00693e;margin-bottom: 4px\">Variable <span style=\"font-size: 13px;font-weight: 400;color: #666\">\/ see pricing table below<\/span><\/div>\n<div style=\"font-size: 12px;color: #999;margin-bottom: 12px\">&nbsp;<\/div>\n<ul style=\"margin: 0;padding-left: 20px\">\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">FASTQ files are stored on Dartmouth's DartFS storage system, accessible to the entire Dartmouth community. Please review our <a href=\"https:\/\/geiselmed.dartmouth.edu\/gsr\/data-retention-policy\/\">Data Retention Policy<\/a> for details on storage timelines. Contact <a href=\"mailto:researchcomputing@dartmouth.edu\">Research Computing<\/a> for a DartFS account.<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">All kits can be run in single-end or paired-end configurations. Sequencing can be purchased as entire flow cells or on a per-read basis for shared runs.<\/li>\n<li style=\"font-size: 14px;color: #555;line-height: 1.5\">NovaSeq runs must be purchased as full flow cells. Most common flow cells are shown below; others available upon request.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"border-collapse: collapse;border-spacing: 0;margin: 0 0 24px 0\" width=\"100%\">\n<tbody>\n<tr>\n<td colspan=\"4\" style=\"background: #00693e;color: #fff;padding: 12px 16px;font-size: 16px;font-weight: bold;border-radius: 8px 8px 0 0\">NextSeq 2000<\/td>\n<\/tr>\n<tr style=\"background: #e8f2ee\">\n<th style=\"padding: 10px 16px;text-align: left;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Flow Cell<\/th>\n<th style=\"padding: 10px 16px;text-align: center;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Reads<\/th>\n<th style=\"padding: 10px 16px;text-align: center;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Cycles<\/th>\n<th style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Price<\/th>\n<\/tr>\n<tr>\n<td rowspan=\"3\" style=\"padding: 10px 16px;font-weight: bold;color: #333;vertical-align: middle;border-right: 1px solid #eee;border-bottom: 2px solid #ddd\">P1<\/td>\n<td rowspan=\"3\" style=\"padding: 10px 16px;text-align: center;color: #555;vertical-align: middle;border-right: 1px solid #eee;border-bottom: 2px solid #ddd\">120M<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 1px solid #eee\">100<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">$1,181.38<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 1px solid #eee\">300<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">$1,415.88<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 2px solid #ddd\">600<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 2px solid #ddd\">$2,005.13<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"3\" style=\"padding: 10px 16px;font-weight: bold;color: #333;vertical-align: middle;border-right: 1px solid #eee;border-bottom: 2px solid #ddd\">P2<\/td>\n<td rowspan=\"3\" style=\"padding: 10px 16px;text-align: center;color: #555;vertical-align: middle;border-right: 1px solid #eee;border-bottom: 2px solid #ddd\">500M<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 1px solid #eee\">100<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">$1,507.57<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 1px solid #eee\">300<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">$1,799.78<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 2px solid #ddd\">600<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 2px solid #ddd\">$3,533.63<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\" style=\"padding: 10px 16px;font-weight: bold;color: #333;vertical-align: middle;border-right: 1px solid #eee;border-bottom: 2px solid #ddd\">P3<\/td>\n<td rowspan=\"2\" style=\"padding: 10px 16px;text-align: center;color: #555;vertical-align: middle;border-right: 1px solid #eee;border-bottom: 2px solid #ddd\">1.3B<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 1px solid #eee\">100<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">$2,105.22<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 2px solid #ddd\">300<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 2px solid #ddd\">$3,113.77<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\" style=\"padding: 10px 16px;font-weight: bold;color: #333;vertical-align: middle;border-right: 1px solid #eee\">P4<\/td>\n<td rowspan=\"2\" style=\"padding: 10px 16px;text-align: center;color: #555;vertical-align: middle;border-right: 1px solid #eee\">2B<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 1px solid #eee\">100<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">$2,810.13<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;text-align: center;color: #555\">300<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333\">$4,285.23<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"border-collapse: collapse;border-spacing: 0;margin: 0 0 24px 0\" width=\"100%\">\n<tbody>\n<tr>\n<td colspan=\"4\" style=\"background: #00693e;color: #fff;padding: 12px 16px;font-size: 16px;font-weight: bold;border-radius: 8px 8px 0 0\">NovaSeq 6000<\/td>\n<\/tr>\n<tr style=\"background: #e8f2ee\">\n<th style=\"padding: 10px 16px;text-align: left;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Flow Cell<\/th>\n<th style=\"padding: 10px 16px;text-align: center;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Reads<\/th>\n<th style=\"padding: 10px 16px;text-align: center;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Cycles<\/th>\n<th style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Price<\/th>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;font-weight: bold;color: #333;border-right: 1px solid #eee;border-bottom: 1px solid #eee\">S2<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-right: 1px solid #eee;border-bottom: 1px solid #eee\">3.3B<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 1px solid #eee\">300<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">$7,921.80<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;font-weight: bold;color: #333;border-right: 1px solid #eee\">S4<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-right: 1px solid #eee\">8.5B<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555\">300<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333\">$11,742.89<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<table style=\"border-collapse: collapse;border-spacing: 0;margin: 0 0 24px 0\" width=\"100%\">\n<tbody>\n<tr>\n<td colspan=\"3\" style=\"background: #00693e;color: #fff;padding: 12px 16px;font-size: 16px;font-weight: bold;border-radius: 8px 8px 0 0\">Shared Runs (Per-Read Pricing)<\/td>\n<\/tr>\n<tr style=\"background: #e8f2ee\">\n<th style=\"padding: 10px 16px;text-align: left;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Option<\/th>\n<th style=\"padding: 10px 16px;text-align: center;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Reads<\/th>\n<th style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Price<\/th>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">10M reads<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 1px solid #eee\">10M<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">$53.33<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">50M reads<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 1px solid #eee\">50M<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">$151.81<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;font-weight: bold;color: #333\">250M reads<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555\">250M<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333\">$803.50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"font-size: 13px;color: #555;font-style: italic;margin: -16px 0 24px 4px\">Shared run prices are based on partial NextSeq 2000 P4 100-cycle run costs. Contact us about partial runs for 300-cycle configurations.<\/p>\n<table style=\"border-collapse: collapse;border-spacing: 0;margin: 0 0 24px 0\" width=\"100%\">\n<tbody>\n<tr>\n<td colspan=\"4\" style=\"background: #00693e;color: #fff;padding: 12px 16px;font-size: 16px;font-weight: bold;border-radius: 8px 8px 0 0\">MiniSeq<\/td>\n<\/tr>\n<tr style=\"background: #e8f2ee\">\n<th style=\"padding: 10px 16px;text-align: left;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Kit<\/th>\n<th style=\"padding: 10px 16px;text-align: center;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Reads<\/th>\n<th style=\"padding: 10px 16px;text-align: center;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Cycles<\/th>\n<th style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #00693e;border-bottom: 1px solid #ddd\">Price<\/th>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;font-weight: bold;color: #333;border-right: 1px solid #eee;border-bottom: 2px solid #ddd\">Mid Output<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-right: 1px solid #eee;border-bottom: 2px solid #ddd\">8M<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 2px solid #ddd\">300<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 2px solid #ddd\">$957.52<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"3\" style=\"padding: 10px 16px;font-weight: bold;color: #333;vertical-align: middle;border-right: 1px solid #eee\">High Output<\/td>\n<td rowspan=\"3\" style=\"padding: 10px 16px;text-align: center;color: #555;vertical-align: middle;border-right: 1px solid #eee\">25M<\/td>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 1px solid #eee\">75<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">$1,301.80<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;text-align: center;color: #555;border-bottom: 1px solid #eee\">150<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333;border-bottom: 1px solid #eee\">$1,477.52<\/td>\n<\/tr>\n<tr>\n<td style=\"padding: 10px 16px;text-align: center;color: #555\">300<\/td>\n<td style=\"padding: 10px 16px;text-align: right;font-weight: bold;color: #333\">$2,211.53<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"font-size: 13px;color: #666;font-style: italic;line-height: 1.5\">Prices shown reflect internal Dartmouth rates for FY2027. External users and those with large projects should contact the core directly for pricing information. Actual prices may vary based on batch size.<\/p>\n<p style=\"font-size: 13px;color: #555;line-height: 1.5\">All services provided by the GMBSR are supported by an NCI Cancer Center Support Grant (5P30CA023108) which MUST be cited in any publications or other materials using data generated in the core.<\/p>\n<p style=\"font-size: 13px;color: #555;line-height: 1.5\">Single cell sequencing services are provided through the Single Cell Genomics Core and are subsidized by a COBRE grant (P20GM130454). This grant MUST be cited in all publications and materials using single cell data generated in the core.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Extraction Nucleic Acid QC RNA-seq DNA-seq Single Cell Spatial Microarray Sequencing The GMBSR provides Illumina next-generation sequencing services. Prices include library normalization, pooling, sequencing, and raw data storage. Use our Cost Estimator Tool for detailed project pricing. Illumina Sequencing Variable \/ see pricing table below &nbsp; FASTQ files are stored [\u2026] <\/p>\n<div class=\"clear\"><\/div>\n<p><a class=\"more_link clearfix\" href=\"https:\/\/geiselmed.dartmouth.edu\/gsr\/sequencing\/\" rel=\"nofollow\">Read More<\/a><\/p>\n","protected":false},"author":145,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-629","page","type-page","status-publish","hentry","author-145"],"jetpack_shortlink":"https:\/\/wp.me\/PbaUZP-a9","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/pages\/629","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/users\/145"}],"replies":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/comments?post=629"}],"version-history":[{"count":16,"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/pages\/629\/revisions"}],"predecessor-version":[{"id":881,"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/pages\/629\/revisions\/881"}],"wp:attachment":[{"href":"https:\/\/geiselmed.dartmouth.edu\/gsr\/wp-json\/wp\/v2\/media?parent=629"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}