Visium Tissue Optimization<\/h3>\n$1,733.66 \/ sample<\/span><\/div>\n <\/div>\n\n- Necessary step prior to performing the Visium: Fresh Frozen assay.<\/li>\n
- In collaboration with Pathology, 7x10um tissue sections are cut onto Visium Tissue Optimization slides and a time course is performed to determine the optimal permeabilization time.<\/li>\n
- A separate optimization run should be performed for each tissue type. It is also recommended to separately optimize healthy vs diseased samples.<\/li>\n<\/ul>\n<\/td>\n
\nVisium Fresh Frozen<\/h3>\n$7,004.58 \/ slide<\/span><\/div>\n <\/div>\n\n- Preparation of tissue sections for spatial transcriptomics using the 10x Genomics Visium assay for fresh frozen tissues.<\/li>\n
- In collaboration with Pathology, 10um sections are cut and placed on the Visium slide (4 capture areas per slide). H&E staining and imaging (20x) are performed in the Single Cell Genomics Core. Transcripts are detected using a PolyA-based approach.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
<\/div>\n
- \n
- Necessary step prior to performing the Visium: Fresh Frozen assay.<\/li>\n
- In collaboration with Pathology, 7x10um tissue sections are cut onto Visium Tissue Optimization slides and a time course is performed to determine the optimal permeabilization time.<\/li>\n
- A separate optimization run should be performed for each tissue type. It is also recommended to separately optimize healthy vs diseased samples.<\/li>\n<\/ul>\n<\/td>\n
\n Visium Fresh Frozen<\/h3>\n
$7,004.58 \/ slide<\/span><\/div>\n<\/div>\n- \n
- Preparation of tissue sections for spatial transcriptomics using the 10x Genomics Visium assay for fresh frozen tissues.<\/li>\n
- In collaboration with Pathology, 10um sections are cut and placed on the Visium slide (4 capture areas per slide). H&E staining and imaging (20x) are performed in the Single Cell Genomics Core. Transcripts are detected using a PolyA-based approach.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
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