{"id":623,"date":"2024-05-20T18:25:36","date_gmt":"2024-05-20T18:25:36","guid":{"rendered":"https:\/\/geiselmed.dartmouth.edu\/gsr\/?page_id=623"},"modified":"2026-04-28T14:46:43","modified_gmt":"2026-04-28T14:46:43","slug":"single-cell-genomics","status":"publish","type":"page","link":"https:\/\/geiselmed.dartmouth.edu\/gsr\/single-cell-genomics\/","title":{"rendered":"Single Cell Genomics"},"content":{"rendered":"\n\n\n
Extraction<\/a><\/td>\nNucleic Acid QC<\/a><\/td>\nRNA-seq<\/a><\/td>\nDNA-seq<\/a><\/td>\nSingle Cell<\/td>\nSpatial<\/a><\/td>\nMicroarray<\/a><\/td>\nSequencing<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Single cell genomics experiments are complex and require tight coordination between the researcher and our core. Consultation with core staff is required before projects are accepted and is provided free-of-charge. Services listed include the cost of sample QC using an automated fluorescent cell counter (Nexcellom K2) as well as all downstream library preparation and QC steps.<\/p>\n

For all 10x genomics workflows, a \"sample\" is equivalent to one lane on the 10x instrument. Each lane can capture between 100-20,000 cells, depending on the concentration loaded onto the instrument.<\/p>\n

Use our Cost Estimator Tool<\/a> for detailed project pricing.<\/p>\n\n\n\n
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Sample Fixation for 10x Genomics Flex<\/h3>\n
$164.36 \/ sample<\/span><\/div>\n
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  • Samples are provided as cell suspensions or tissues for fixation following the 10x Genomics Flex protocol.<\/li>\n
  • Cells are stored at -80C in the GMBSR until ready for processing (within 1 year).<\/li>\n<\/ul>\n<\/td>\n

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Tissue Processing for 10x Genomics<\/h3>\n
$268.11 \/ sample<\/span><\/div>\n
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  • Nuclei isolation from fresh\/frozen tissue<\/li>\n
  • Tissue dissociation into single cell suspension (off-the-shelf protocols available for some tissues; those requiring optimization may incur additional charges)<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n
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    10x 3'\/5' RNA-seq<\/h3>\n
    $2,159.36 \/ sample<\/span><\/div>\n
    Based on 2 sample batch<\/div>\n
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    • Uses the 10x Genomics workflows for 3' and 5'-End RNA-seq. For standard gene expression, 3' RNA-seq is the standard in the field. 5' RNA-seq is required where TCR and\/or BCR (V(D)J) sequencing is performed.<\/li>\n
    • Up to 96 unique barcodes are available for pooled sequencing.<\/li>\n
    • Recommended sequencing depth is 30,000 reads per cell.<\/li>\n<\/ul>\n<\/td>\n

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    10x 3'\/5' RNA-seq OCM<\/h3>\n
    $3,431.66 \/ lane<\/span><\/div>\n
    $857.92\/sample · 1 lane of 4 samples<\/div>\n