{"id":619,"date":"2024-05-20T18:24:42","date_gmt":"2024-05-20T18:24:42","guid":{"rendered":"https:\/\/geiselmed.dartmouth.edu\/gsr\/?page_id=619"},"modified":"2026-04-28T14:46:43","modified_gmt":"2026-04-28T14:46:43","slug":"rna-seq","status":"publish","type":"page","link":"https:\/\/geiselmed.dartmouth.edu\/gsr\/rna-seq\/","title":{"rendered":"RNA-seq"},"content":{"rendered":"\n\n\n
Extraction<\/a><\/td>\nNucleic Acid QC<\/a><\/td>\nRNA-seq<\/td>\nDNA-seq<\/a><\/td>\nSingle Cell<\/a><\/td>\nSpatial<\/a><\/td>\nMicroarray<\/a><\/td>\nSequencing<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

To assist users in determining the most appropriate RNA-seq workflow for their project we have created a tool that can be accessed HERE<\/a>. It is always recommended to consult with core staff (free-of-charge) before finalizing an RNA-seq experimental design.<\/p>\n\n\n\n\n
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Ribodepletion<\/h3>\n
$171.04 \/ sample<\/span><\/div>\n
Based on 30 sample batch<\/div>\n
    \n
  • Full-length coverage for mRNAs and lncRNAs<\/li>\n
  • Ideal for full transcriptome characterization<\/li>\n
  • Only option for prokaryotic samples<\/li>\n
  • Recommended: ~30M reads\/sample<\/li>\n
  • Up to 192 samples per pool<\/li>\n<\/ul>\n<\/td>\n

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3' End-Counting<\/h3>\n
$93.53 \/ sample<\/span><\/div>\n
Based on 30 sample batch<\/div>\n
    \n
  • Cost-effective differential expression<\/li>\n
  • Poly-A capture with 3' end amplification<\/li>\n
  • Best for annotated genomes (Human, Mouse, Rat)<\/li>\n
  • Cannot identify splice isoforms<\/li>\n
  • Recommended: ~10M reads\/sample<\/li>\n
  • Up to 96 samples per pool<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n

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miRNA \/ smRNA<\/h3>\n
$194.45 \/ sample<\/span><\/div>\n
Based on 30 sample batch<\/div>\n
    \n
  • Captures miRNAs\/smRNAs too small for standard workflows<\/li>\n
  • Direct ligation with UMIs to minimize PCR bias<\/li>\n
  • Recommended: 1-10M reads\/sample<\/li>\n
  • Up to 96 samples per pool<\/li>\n<\/ul>\n<\/td>\n

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Low Input RNA-seq<\/h3>\n
$156.88 \/ sample<\/span><\/div>\n
Based on 30 sample batch<\/div>\n