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Henry N. Higgs, PhD

Professor of Biochemistry and Cell Biology

Biochemistry and Cell Biology

Lafayette College, BA 1987U. Washington, PHD 1996

Molecular and Cellular Biology Graduate Programs

Contact Information:

Dartmouth Medical School
HB 7200
Hanover NH 03755

Professional Interests:

Microvilli cover the surface of circulating blood lymphocytes. Lymphocytes use these finger-like projections to segregate adhesion receptors, as certain receptors localize to microvilli while others are excluded from them. Adhesion receptor segregation is crucial because cells use these receptors sequentially when moving from the bloodsteam to specific tissues. Little is known about the architecture of microvilli, nor about their relationship to similar structures from other cells. My lab will identify protein components crucial to microvillar structure, test how mutation of these proteins affect microvillar structure and function, and characterize how these proteins function biochemically and biophysically.

Selected Publications:


Function-Oriented Studies Targeting Pectenotoxin 2: Synthesis of the GH-Ring System and a Structurally Simplified Macrolactone.
O'Rourke NF, A M, Higgs HN, Eastman A, Micalizio GC
Org Lett. 2017 Sep 13; doi: 10.1021/acs.orglett.7b02435. Epub 2017 Sep 13.
PMID: 28901150

Calcium-mediated actin reset (CaAR) mediates acute cell adaptations.
Wales P, Schuberth CE, Aufschnaiter R, Fels J, Garcia-Aguilar I, Janning A, Dlugos CP, Schafer-Herte M, Klingner C, Walte M, Kuhlmann J, Menis E, Hockaday Kang L, Maier KC, Hou W, Russo A, Higgs HN, Pavenstadt H, Vogl T, Roth J, Qualmann B, Kessels MM, Martin DE, Mulder B, Wedlich-Soldner R
Elife. 2016 Dec 6;5 pii: e19850. doi: 10.7554/eLife.19850. Epub 2016 Dec 6.
PMID: 27919320

Actin filaments as dynamic reservoirs for Drp1 recruitment.
Hatch AL, Ji WK, Merrill RA, Strack S, Higgs HN
Mol Biol Cell. 2016 Oct 15;27(20):3109-3121. Epub 2016 Aug 24.
PMID: 27559132

Mice with mutant Inf2 show impaired podocyte and slit diaphragm integrity in response to protamine-induced kidney injury.
Subramanian B, Sun H, Yan P, Charoonratana VT, Higgs HN, Wang F, Lai KV, Valenzuela DM, Brown EJ, Schlondorff JS, Pollak MR
Kidney Int. 2016 Aug;90(2):363-372. doi: 10.1016/j.kint.2016.04.020. Epub 2016 Jun 24.
PMID: 27350175

Actin filaments target the oligomeric maturation of the dynamin GTPase Drp1 to mitochondrial fission sites.
Ji WK, Hatch AL, Merrill RA, Strack S, Higgs HN
Elife. 2015 Nov 26;4:e11553. doi: 10.7554/eLife.11553. Epub 2015 Nov 26.
PMID: 26609810

Cell type-dependent mechanisms for formin-mediated assembly of filopodia.
Young LE, Heimsath EG, Higgs HN
Mol Biol Cell. 2015 Dec 15;26(25):4646-59. doi: 10.1091/mbc.E15-09-0626. Epub 2015 Oct 7.
PMID: 26446836

Assembly and turnover of short actin filaments by the formin INF2 and profilin.
Gurel PS, A M, Guo B, Shu R, Mierke DF, Higgs HN
J Biol Chem. 2015 Sep 11;290(37):22494-506. doi: 10.1074/jbc.M115.670166. Epub 2015 Jun 29.
PMID: 26124273

The formin FMNL3 assembles plasma membrane protrusions that participate in cell-cell adhesion.
Gauvin TJ, Young LE, Higgs HN
Mol Biol Cell. 2015 Feb 1;26(3):467-77. doi: 10.1091/mbc.E14-07-1247. Epub 2014 Nov 26.
PMID: 25428984

Novel roles for actin in mitochondrial fission.
Hatch AL, Gurel PS, Higgs HN
J Cell Sci. 2014 Nov 1;127(Pt 21):4549-60. doi: 10.1242/jcs.153791. Epub 2014 Sep 12.
PMID: 25217628

Monitoring ATP hydrolysis and ATPase inhibitor screening using (1)H NMR.
Guo B, Gurel PS, Shu R, Higgs HN, Pellegrini M, Mierke DF
Chem Commun (Camb). 2014 Oct 18;50(81):12037-9. doi: 10.1039/c4cc04399e.
PMID: 25170530