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Henry N. Higgs, PhD

Professor of Biochemistry and Cell Biology

Biochemistry and Cell Biology

Lafayette College, BA 1987U. Washington, PHD 1996

Molecular and Cellular Biology Graduate Programs

Contact Information:

Dartmouth Medical School
HB 7200
Hanover NH 03755

Professional Interests:

Microvilli cover the surface of circulating blood lymphocytes. Lymphocytes use these finger-like projections to segregate adhesion receptors, as certain receptors localize to microvilli while others are excluded from them. Adhesion receptor segregation is crucial because cells use these receptors sequentially when moving from the bloodsteam to specific tissues. Little is known about the architecture of microvilli, nor about their relationship to similar structures from other cells. My lab will identify protein components crucial to microvillar structure, test how mutation of these proteins affect microvillar structure and function, and characterize how these proteins function biochemically and biophysically.

Selected Publications:


Receptor-mediated Drp1 oligomerization on endoplasmic reticulum.
Ji WK, Chakrabarti R, Fan X, Schoenfeld L, Strack S, Higgs HN
J Cell Biol. 2017 Dec 4;216(12):4123-4139. doi: 10.1083/jcb.201610057. Epub 2017 Nov 20.
PMID: 29158231

INF2-mediated actin polymerization at the ER stimulates mitochondrial calcium uptake, inner membrane constriction, and division.
Chakrabarti R, Ji WK, Stan RV, de Juan Sanz J, Ryan TA, Higgs HN
J Cell Biol. 2018 Jan 2;217(1):251-268. doi: 10.1083/jcb.201709111. Epub 2017 Nov 15.
PMID: 29142021

Actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes.
Fritz-Laylin LK, Riel-Mehan M, Chen BC, Lord SJ, Goddard TD, Ferrin TE, Nicholson-Dykstra SM, Higgs H, Johnson GT, Betzig E, Mullins RD
Elife. 2017 Sep 26;6 pii: e26990. doi: 10.7554/eLife.26990. Epub 2017 Sep 26.
PMID: 28948912

Function-Oriented Studies Targeting Pectenotoxin 2: Synthesis of the GH-Ring System and a Structurally Simplified Macrolactone.
O'Rourke NF, A M, Higgs HN, Eastman A, Micalizio GC
Org Lett. 2017 Oct 6;19(19):5154-5157. doi: 10.1021/acs.orglett.7b02435. Epub 2017 Sep 13.
PMID: 28901150

Calcium-mediated actin reset (CaAR) mediates acute cell adaptations.
Wales P, Schuberth CE, Aufschnaiter R, Fels J, Garcia-Aguilar I, Janning A, Dlugos CP, Schafer-Herte M, Klingner C, Walte M, Kuhlmann J, Menis E, Hockaday Kang L, Maier KC, Hou W, Russo A, Higgs HN, Pavenstadt H, Vogl T, Roth J, Qualmann B, Kessels MM, Martin DE, Mulder B, Wedlich-Soldner R
Elife. 2016 Dec 6;5 pii: e19850. doi: 10.7554/eLife.19850. Epub 2016 Dec 6.
PMID: 27919320

Actin filaments as dynamic reservoirs for Drp1 recruitment.
Hatch AL, Ji WK, Merrill RA, Strack S, Higgs HN
Mol Biol Cell. 2016 Oct 15;27(20):3109-3121. Epub 2016 Aug 24.
PMID: 27559132

Mice with mutant Inf2 show impaired podocyte and slit diaphragm integrity in response to protamine-induced kidney injury.
Subramanian B, Sun H, Yan P, Charoonratana VT, Higgs HN, Wang F, Lai KV, Valenzuela DM, Brown EJ, Schlondorff JS, Pollak MR
Kidney Int. 2016 Aug;90(2):363-372. doi: 10.1016/j.kint.2016.04.020. Epub 2016 Jun 24.
PMID: 27350175

Actin filaments target the oligomeric maturation of the dynamin GTPase Drp1 to mitochondrial fission sites.
Ji WK, Hatch AL, Merrill RA, Strack S, Higgs HN
Elife. 2015 Nov 26;4:e11553. doi: 10.7554/eLife.11553. Epub 2015 Nov 26.
PMID: 26609810

Cell type-dependent mechanisms for formin-mediated assembly of filopodia.
Young LE, Heimsath EG, Higgs HN
Mol Biol Cell. 2015 Dec 15;26(25):4646-59. doi: 10.1091/mbc.E15-09-0626. Epub 2015 Oct 7.
PMID: 26446836

Assembly and turnover of short actin filaments by the formin INF2 and profilin.
Gurel PS, A M, Guo B, Shu R, Mierke DF, Higgs HN
J Biol Chem. 2015 Sep 11;290(37):22494-506. doi: 10.1074/jbc.M115.670166. Epub 2015 Jun 29.
PMID: 26124273