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Charles K. Barlowe, PhD

Title(s):
Chair and Professor of Biochemistry and Cell Biology
James C. Chilcott '20 Professor in Pharmaceutical Sciences

Additional Titles/Positions/Affiliations:
James C. Chilcott 1920 Professor

Department(s):
Biochemistry and Cell Biology

Education:
College of William & Mary, B.S. 1983
University of Texas at Austin, Ph.D. 1990

After postdoctoral work at University of California-Berkeley, Dr. Barlowe joined the Department of Biochemistry and Cell Biology at Geisel School of Medicine in 1994

Programs:
Molecular and Cellular Biology Graduate Programs

Websites:
http://geiselmed.dartmouth.edu/barlowe/

Contact Information:

Geisel School of Medicine
Department of Biochemistry and Cell Biology
HB 7200
Hanover NH 03755

Office: Remsen 414
Phone: 650-6516


Professional Interests:

Our current focus is on molecular mechanisms of protein and lipid trafficking between the endoplasmic reticulum (ER) and the Golgi complex in. Several of the genes involved in this process have been identified and their function may be assessed in cell-free assays that reproduce transport. Through a combined genetic and biochemical approach, the mechanisms underlying secretory cargo selection and vesicle budding from the ER as well as vesicle targeting and fusion with the Golgi complex are under study. This intracellular transport step is essential for cellular function and our studies are basic for understanding numerous health related issues including cholesterol regulation, cystic fibrosis and diabetes.

Biography:

NIH Biosketch


Selected Publications:

 

Twenty-five years after coat protein complex II.
Barlowe C
Mol Biol Cell. 2020 Jan 1;31(1):3-6. doi: 10.1091/mbc.E19-11-0621.
PMID: 31887067

Molecular dissection of the Erv41-Erv46 retrograde receptor reveals a conserved cysteine-rich region in Erv46 required for retrieval activity.
Keiser KJ, Barlowe C
Mol Biol Cell. 2020 Feb 1;31(3):209-220. doi: 10.1091/mbc.E19-08-0484. Epub 2019 Dec 11.
PMID: 31825724

Conserved juxtamembrane domains in the yeast golgin Coy1 drive assembly of a megadalton-sized complex and mediate binding to tethering and SNARE proteins.
Anderson NS, Barlowe C
J Biol Chem. 2019 Jun 21;294(25):9690-9705. doi: 10.1074/jbc.RA119.008107. Epub 2019 May 9.
PMID: 31073031

Lysophospholipids Facilitate COPII Vesicle Formation.
Melero A, Chiaruttini N, Karashima T, Riezman I, Funato K, Barlowe C, Riezman H, Roux A
Curr Biol. 2018 Jun 18;28(12):1950-1958.e6. doi: 10.1016/j.cub.2018.04.076. Epub 2018 Jun 7.
PMID: 29887313

The Golgin protein Coy1 functions in intra-Golgi retrograde transport and interacts with the COG complex and Golgi SNAREs.
Anderson NS, Mukherjee I, Bentivoglio CM, Barlowe C
Mol Biol Cell. 2017 Aug 9; pii: mbc.E17-03-0137. doi: 10.1091/mbc.E17-03-0137. Epub 2017 Aug 9.
PMID: 28794270

Cargo Capture and Bulk Flow in the Early Secretory Pathway.
Barlowe C, Helenius A
Annu Rev Cell Dev Biol. 2016 Oct 6;32:197-222. Epub 2016 Jun 8.
PMID: 27298089

Overexpression of Sly41 suppresses COPII vesicle-tethering deficiencies by elevating intracellular calcium levels.
Mukherjee I, Barlowe C
Mol Biol Cell. 2016 May 15;27(10):1635-49. doi: 10.1091/mbc.E15-10-0704. Epub 2016 Mar 30.
PMID: 27030673

Analysis of COPII Vesicles Indicates a Role for the Emp47-Ssp120 Complex in Transport of Cell Surface Glycoproteins.
Margulis NG, Wilson JD, Bentivoglio CM, Dhungel N, Gitler AD, Barlowe C
Traffic. 2016 Mar;17(3):191-210. doi: 10.1111/tra.12356. Epub 2016 Jan 10.
PMID: 26650540

Examination of Sec22 Homodimer Formation and Role in SNARE-dependent Membrane Fusion.
Flanagan JJ, Mukherjee I, Barlowe C
J Biol Chem. 2015 Apr 24;290(17):10657-66. doi: 10.1074/jbc.M114.626911. Epub 2015 Mar 6.
PMID: 25750128

Membrane trafficking: ER export encounters dualism.
Barlowe C
Curr Biol. 2015 Feb 16;25(4):R151-3. doi: 10.1016/j.cub.2015.01.017.
PMID: 25689910

View more publications on PubMed