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H Robert Frost, PhD

Title(s)
Associate Professor of Biomedical Data Science
Associate Professor of Molecular and Systems Biology

Additional Titles/Positions/Affiliations
Associate Director of Quantitative Biomedical Sciences Graduate Program

Department(s)
Biomedical Data Science
Molecular and Systems Biology

Education
Dartmouth College, Ph.D., 2014
Stanford University, MS, 1995
Stanford University, BS, 1993

Programs
Molecular and Cellular Biology Graduate Programs
Dartmouth Cancer Center
Quantitative Biomedical Sciences

Websites
https://sites.dartmouth.edu/frost

Contact Information

HB 7936
Hanover NH 03755

Office: Rubin 704
Email: Hildreth.R.Frost@Dartmouth.edu


Professional Interests

My research focuses on the development of bioinformatics and biostatistics methods for analyzing high-dimensional genomic data. Areas of statistical interest include dimensionality reduction (e.g., PCA), hypothesis aggregation (e.g., gene set testing), and penalized estimation (e.g., LASSO penalized regression). Areas of biological interest include cell signaling, tissue-specific gene activity, tumor immunology, and cancer prognosis prediction.

Grant Information

NIH grants R35GM146586 and R21CA253408

Courses Taught

QBS 120: Foundations of Biostatistics I, Statistical Theory for the Quantitative Biomedical Sciences


Selected Publications

 

Biological foundation models illuminate annotation blind spots in evolutionarily divergent genomes.
Lanser TB, Caldwell SK, Pacheco GA, Chen JW, Saghaei S, Hassan M, Kronrod M, Wesemann DR, Frost HR
bioRxiv. 2026 May 16; pii: 2026.05.15.724572. doi: 10.64898/2026.05.15.724572. Epub 2026 May 16.
PMID: 42182479

Autoencoder Denoising for Network-Based Spatial Transcriptomics Data with Applications for Cell Signaling Estimation.
Javaid A, Frost HR
Complex Netw Appl. 2026;1266:196-207. doi: 10.1007/978-3-032-16719-4_16. Epub 2026 May 1.
PMID: 42181748

Benchmarking sketching methods on spatial transcriptomics data.
Gingerich IK, Goods BA, Frost HR
Nucleic Acids Res. 2026 May 5;54(9) doi: 10.1093/nar/gkag434.
PMID: 42109248

Analysis of biological networks using Krylov subspace trajectories.
Frost HR
bioRxiv. 2026 Mar 31; pii: 2026.03.29.715092. doi: 10.64898/2026.03.29.715092. Epub 2026 Mar 31.
PMID: 41959399

A Composite interaction Score: Prioritizing cell-cell interactions from single-cell RNA-seq with application to pre-menopausal epithelial barriers.
Kholod O, Bui HM, Frost HR, Goods BA
J Adv Res. 2026 Mar 22; pii: S2090-1232(26)00267-5. doi: 10.1016/j.jare.2026.03.046. Epub 2026 Mar 22.
PMID: 41875947

Antibody responses against bacterial glycans affinity mature and diversify in germinal centers.
Fryer HA, Pitt C, Frost HR, Kandhari N, Byars S, Lim PS, Fettes RL, Lovett RM, Nguyen TT, Chheng K, Caltabiano N, Whitcombe AL, Hamelink J, Andrew D, Lloyd G, Wilson-Boyd B, Slee N, Ballantine J, Vasani S, Girling K, Gubbels L, Levi E, Davies K, Tangye SG, Noonan J, Moreland NJ, Quast I, Robinson MJ, Scally SW, Neeland MR, Shanthikumar S, Osowicki J, Tarlinton DM, Steer AC, Boyle MJ, Hill DL
Immunity. 2026 Mar 10;59(3):666-681.e5. doi: 10.1016/j.immuni.2026.02.006.
PMID: 41812644

Randomized Spatial PCA (RASP): A computationally efficient method for dimensionality reduction of high-resolution spatial transcriptomics data.
Gingerich IK, Goods BA, Frost HR
PLoS Comput Biol. 2025 Dec;21(12):e1013759. doi: 10.1371/journal.pcbi.1013759. Epub 2025 Dec 10.
PMID: 41370353

Revealing tissue architecture through the hypercomplex Fourier analysis of spatial transcriptomics data.
Frost HR
Bioinform Adv. 2025;5(1):vbaf191. doi: 10.1093/bioadv/vbaf191. Epub 2025 Aug 11.
PMID: 41195088

Mouse-Specific Single cell cytokine activity prediction and Estimation (MouSSE).
Javaid A, Frost HR
PLoS Comput Biol. 2025 Sep;21(9):e1013475. doi: 10.1371/journal.pcbi.1013475. Epub 2025 Sep 19.
PMID: 40971965

Benchmarking sketching methods on spatial transcriptomics data.
Gingerich IK, Goods BA, Frost HR
bioRxiv. 2025 Sep 2; pii: 2025.08.26.672376. doi: 10.1101/2025.08.26.672376. Epub 2025 Sep 2.
PMID: 40909701

View more publications on PubMed