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H Robert Frost, PhD

Assistant Professor of Biomedical Data Science
Assistant Professor of Molecular and Systems Biology

Additional Titles/Positions/Affiliations
Associate Director of Quantitative Biomedical Sciences Graduate Program

Biomedical Data Science
Molecular and Systems Biology

Dartmouth College, Ph.D., 2014
Stanford University, MS, 1995
Stanford University, BS, 1993

Molecular and Cellular Biology Graduate Programs
Norris Cotton Cancer Center
Quantitative Biomedical Sciences


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Contact Information

HB 7936
Hanover NH 03755

Office: Rubin 704
Email: Hildreth.R.Frost@Dartmouth.edu

Professional Interests

My research focuses on the development of bioinformatics and biostatistics methods for analyzing high-dimensional genomic data. Areas of statistical interest include dimensionality reduction (e.g., PCA), hypothesis aggregation (e.g., gene set testing), and penalized estimation (e.g., LASSO penalized regression). Areas of biological interest include cell signaling, tissue-specific gene activity, tumor immunology, and cancer prognosis prediction.

Grant Information

NIH grants R35GM146586 and R21CA253408

Courses Taught

QBS 120: Foundations of Biostatistics I, Statistical Theory for the Quantitative Biomedical Sciences

Selected Publications


Cell type-specific interaction analysis using doublets in scRNA-seq.
Schiebout C, Lust H, Huang Y, Frost HR
Bioinform Adv. 2023;3(1):vbad120. doi: 10.1093/bioadv/vbad120. Epub 2023 Sep 6.
PMID: 37745004

STREAK: A supervised cell surface receptor abundance estimation strategy for single cell RNA-sequencing data using feature selection and thresholded gene set scoring.
Javaid A, Frost HR
PLoS Comput Biol. 2023 Aug;19(8):e1011413. doi: 10.1371/journal.pcbi.1011413. Epub 2023 Aug 21.
PMID: 37603589

Characterizing control of memory CD8 T cell differentiation by BTB-ZF transcription factor Zbtb20.
Preiss NK, Kamal Y, Wilkins OM, Li C, Kolling FW 4th, Trask HW, Usherwood YK, Cheng C, Frost HR, Usherwood EJ
Life Sci Alliance. 2023 Sep;6(9) doi: 10.26508/lsa.202201683. Epub 2023 Jul 6.
PMID: 37414528

SPECK: an unsupervised learning approach for cell surface receptor abundance estimation for single-cell RNA-sequencing data.
Javaid A, Frost HR
Bioinform Adv. 2023;3(1):vbad073. doi: 10.1093/bioadv/vbad073. Epub 2023 Jun 13.
PMID: 37359727

Reconstruction Set Test (RESET): a computationally efficient method for single sample gene set testing based on randomized reduced rank reconstruction error.
Frost HR
bioRxiv. 2023 Apr 20; pii: 2023.04.03.535366. doi: 10.1101/2023.04.03.535366. Epub 2023 Apr 20.
PMID: 37066315

Tumor type and cell type-specific gene expression alterations in diverse pediatric central nervous system tumors identified using single nuclei RNA-seq.
Lee MK, Azizgolshani N, Shapiro JA, Nguyen LN, Kolling FW, Zanazzi GJ, Frost HR, Christensen BC
Res Sq. 2023 Feb 23; pii: rs.3.rs-2517703. doi: 10.21203/rs.3.rs-2517703/v1. Epub 2023 Feb 23.
PMID: 36865335

Cell type-specific Interaction Analysis using Doublets in scRNA-seq (CIcADA).
Schiebout C, Lust HE, Huang YH, Frost HR
bioRxiv. 2023 Feb 15; pii: 2023.02.13.528326. doi: 10.1101/2023.02.13.528326. Epub 2023 Feb 15.
PMID: 36824707

Promiscuous evolution of Group A Streptococcal M and M-like proteins.
Frost HR, Guglielmini J, Duchene S, Lacey JA, Sanderson-Smith M, Steer AC, Walker MJ, Botteaux A, Davies MR, Smeesters PR
Microbiology (Reading). 2023 Jan;169(1) doi: 10.1099/mic.0.001280.
PMID: 36748538

Intraoperative plasma proteomic changes in cardiac surgery: In search of biomarkers of post-operative delirium.
Wiredu K, O'Connor S, Naseem H, Brauer BL, Kettenbach AN, Frost HR, Shaefi S, Gerber SA
Proteomics Clin Appl. 2023 Jul;17(4):e2200066. doi: 10.1002/prca.202200066. Epub 2023 Jan 4.
PMID: 36567636

CAMML with the Integration of Marker Proteins (ChIMP).
Schiebout C, Frost HR
Bioinformatics. 2022 Nov 30;38(23):5206-5213. doi: 10.1093/bioinformatics/btac674.
PMID: 36214642

View more publications on PubMed