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H Robert Frost, PhD

Title(s)
Associate Professor of Biomedical Data Science
Associate Professor of Molecular and Systems Biology

Additional Titles/Positions/Affiliations
Associate Director of Quantitative Biomedical Sciences Graduate Program

Department(s)
Biomedical Data Science
Molecular and Systems Biology

Education
Dartmouth College, Ph.D., 2014
Stanford University, MS, 1995
Stanford University, BS, 1993

Programs
Molecular and Cellular Biology Graduate Programs
Dartmouth Cancer Center
Quantitative Biomedical Sciences

Websites
https://sites.dartmouth.edu/frost

Contact Information

HB 7936
Hanover NH 03755

Office: Rubin 704
Email: Hildreth.R.Frost@Dartmouth.edu


Professional Interests

My research focuses on the development of bioinformatics and biostatistics methods for analyzing high-dimensional genomic data. Areas of statistical interest include dimensionality reduction (e.g., PCA), hypothesis aggregation (e.g., gene set testing), and penalized estimation (e.g., LASSO penalized regression). Areas of biological interest include cell signaling, tissue-specific gene activity, tumor immunology, and cancer prognosis prediction.

Grant Information

NIH grants R35GM146586 and R21CA253408

Courses Taught

QBS 120: Foundations of Biostatistics I, Statistical Theory for the Quantitative Biomedical Sciences


Selected Publications

 

Randomized Spatial PCA (RASP): A computationally efficient method for dimensionality reduction of high-resolution spatial transcriptomics data.
Gingerich IK, Goods BA, Frost HR
PLoS Comput Biol. 2025 Dec;21(12):e1013759. doi: 10.1371/journal.pcbi.1013759. Epub 2025 Dec 10.
PMID: 41370353

Revealing tissue architecture through the hypercomplex Fourier analysis of spatial transcriptomics data.
Frost HR
Bioinform Adv. 2025;5(1):vbaf191. doi: 10.1093/bioadv/vbaf191. Epub 2025 Aug 11.
PMID: 41195088

Mouse-Specific Single cell cytokine activity prediction and Estimation (MouSSE).
Javaid A, Frost HR
PLoS Comput Biol. 2025 Sep;21(9):e1013475. doi: 10.1371/journal.pcbi.1013475. Epub 2025 Sep 19.
PMID: 40971965

Benchmarking sketching methods on spatial transcriptomics data.
Gingerich IK, Goods BA, Frost HR
bioRxiv. 2025 Sep 2; pii: 2025.08.26.672376. doi: 10.1101/2025.08.26.672376. Epub 2025 Sep 2.
PMID: 40909701

Gene set optimization for cancer transcriptomics using sparse principal component analysis.
Frost HR
bioRxiv. 2025 May 26; pii: 2025.05.21.655279. doi: 10.1101/2025.05.21.655279. Epub 2025 May 26.
PMID: 40661635

Cell signaling characterization for spatial transcriptomics (ST) data using network analysis.
Javaid A, Frost HR
Complex Netw Appl XIII (2024). 2025;1189:394-405. doi: 10.1007/978-3-031-82435-7_32. Epub 2025 Mar 28.
PMID: 40510773

Gene set optimization for single cell transcriptomics.
Frost HR
Comput Intell Methods Bioinform Biostat. 2025;15276:183-195. doi: 10.1007/978-3-031-89704-7_14. Epub 2025 May 15.
PMID: 40487192

Antibody responses against bacterial glycans affinity mature and diversify in germinal centers.
Fryer HA, Pitt C, Frost HR, Kandhari N, Byars S, Lim PS, Nguyen TT, Chheng K, Caltabiano N, Whitcombe AL, Hamelink J, Andrew D, Lloyd G, Wilson-Boyd B, Slee N, Ballantine J, Vasani S, Girling K, Gubbels L, Levi E, Davies K, Tangye S, Noonan J, Moreland NJ, Quast I, Robinson MJ, Scally SW, Neeland M, Shanthikumar S, Osowicki J, Tarlinton DM, Steer AC, Boyle MJ, Hill DL
bioRxiv. 2025 Mar 31; pii: 2025.03.26.645614. doi: 10.1101/2025.03.26.645614. Epub 2025 Mar 31.
PMID: 40236127

Fibrinogen-binding M-related proteins facilitate the recruitment of plasminogen by Streptococcus pyogenes.
Proctor EJ, Frost HR, Mantri B, Satapathy S, Botquin G, Gorman J, De Oliveira DMP, McArthur J, Davies MR, Tolun G, Botteaux A, Smeesters P, Sanderson-Smith M
Protein Sci. 2025 Apr;34(4):e70078. doi: 10.1002/pro.70078.
PMID: 40100134

Randomized Spatial PCA (RASP): a computationally efficient method for dimensionality reduction of high-resolution spatial transcriptomics data.
Gingerich IK, Goods BA, Frost HR
Res Sq. 2025 Feb 20; pii: rs.3.rs-6050441. doi: 10.21203/rs.3.rs-6050441/v1. Epub 2025 Feb 20.
PMID: 40034439

View more publications on PubMed