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Alix Ashare, MD, PhD

Title(s):
Associate Professor of Medicine
Associate Professor of Microbiology and Immunology

Department(s):
Medicine
Microbiology and Immunology

Education:
Vanderbilt University School of Medicine, MD 1999
University of Iowa College of Medicine, PhD 2008

Programs:
Molecular and Cellular Biology Graduate Programs

Websites:
https://geiselmed.dartmouth.edu/ashare/

Contact Information:

1 Medical Center Dr
Lebanon NH 03756

Office: 508E Borwell
Phone: 603-650-5533
Fax: 603-650-0580
Email: Alix.Ashare@dartmouth.edu


Professional Interests:

My research focuses on the role of lung macrophages in the development of inflammatory lung diseases. Currently my laboratory is investigating the potential mechanisms of increased lung inflammation in patients with cystic fibrosis (CF) and asthma, with an emphasis on the investigation of impaired alveolar macrophage function in the patient populations.

A major focus of my laboratory is the investigation of how the metabolic state of lung macrophages impacts their phenotype and function and how these changes in the immune cell may contribute to chronic inflammation in CF and asthma. We are also interested in how new medication aimed at treating the underlying defect in CF impact macrophage populations. We are currently funded by the Cystic Fibrosis Foundation to investigate the metabolic state and inflammatory profile of macrophages isolated from CF patients treated with highly effective modulator therapy. Another area of focus involves studying the interaction between bacterial pathogens and host innate immune cells in the CF lung.

Rotations and Thesis Projects:

Project 1: One potential project involves looking at the effects of outer membrane vesicles released by bacteria on the inflammatory response of human macrophages. This project will compare different strains of bacteria as well as treatment with various antibiotics to investigate the impact on the macrophage inflammatory response.

Project 2: This project involves looking at the effect of chronic e-cigarette exposure on lipid homeostasis in lung macrophages.

Grant Information:

Macrophage Pathogen Interactions in Regional CF Lung Inflammation (NIH/NHLBI)

The Impact of CFTR Modulator Treatment on CF Lung Macrophage Inflammation (CF Foundation)

The Role of Lung Macrophage Metabolic State in CF Lung Inflammation (CF Foundation)

Courses Taught:

CF: From Bedside and Bench, and Back Again
Scientific Basis of Medicine: Respiratory Section

Biography:

Dr. Ashare received her B.S. from Vanderbilt University in 1994, her M.D. from Vanderbilt University School of Medicine in 1999, and her Ph.D. in Translational Biomedicine from the University of Iowa in 2008. Following Medical School, she completed an Internal Medicine Residency and Pulmonary & Critical Care Fellowship at the University of Iowa Hospitals and Clinics in 2002 and 2005, respectively. In 2005, she joined the faculty at the University of Iowa. She moved her clinical and research program to Dartmouth in 2009.


Selected Publications:

 

Model Systems to Study the Chronic, Polymicrobial Infections in Cystic Fibrosis: Current Approaches and Exploring Future Directions.
O'Toole GA, Crabbe A, Kummerli R, LiPuma JJ, Bomberger JM, Davies JC, Limoli D, Phelan VV, Bliska JB, DePas WH, Dietrich LE, Hampton TH, Hunter R, Khursigara CM, Price-Whelan A, Ashare A, Cramer RA, Goldberg JB, Harrison F, Hogan DA, Henson MA, Madden DR, Mayers JR, Nadell C, Newman D, Prince A, Rivett DW, Schwartzman JD, Schultz D, Sheppard DC, Smyth AR, Spero MA, Stanton BA, Turner PE, van der Gast C, Whelan FJ, Whitaker R, Whiteson K
mBio. 2021 Sep 21;:e0176321. doi: 10.1128/mBio.01763-21. Epub 2021 Sep 21.
PMID: 34544277

Aspergillus fumigatus In-Host HOG Pathway Mutation for Cystic Fibrosis Lung Microenvironment Persistence.
Ross BS, Lofgren LA, Ashare A, Stajich JE, Cramer RA
mBio. 2021 Aug 31;12(4):e0215321. doi: 10.1128/mBio.02153-21. Epub 2021 Aug 31.
PMID: 34465017

Healthy versus inflamed lung environments differentially effect MSCs.
Rolandsson Enes S, Hampton TH, Barua J, McKenna DH, Dos Santos CC, Amiel E, Ashare A, Liu KD, Krasnodembskaya AD, English K, Stanton BA, Rocco PRM, Matthay MA, Weiss DJ
Eur Respir J. 2021 Apr 1; pii: 2004149. doi: 10.1183/13993003.04149-2020. Epub 2021 Apr 1.
PMID: 33795318

Balancing Positive and Negative Selection: In Vivo Evolution of Candida lusitaniae MRR1.
Demers EG, Stajich JE, Ashare A, Occhipinti P, Hogan DA
mBio. 2021 Mar 30;12(2) pii: e03328-20. doi: 10.1128/mBio.03328-20. Epub 2021 Mar 30.
PMID: 33785623

Safety of research bronchoscopy with BAL in stable adult patients with cystic fibrosis.
Aridgides D, Dessaint J, Atkins G, Carroll J, Ashare A
PLoS One. 2021;16(1):e0245696. doi: 10.1371/journal.pone.0245696. Epub 2021 Jan 22.
PMID: 33481845

CF monocyte-derived macrophages have an attenuated response to extracellular vesicles secreted by airway epithelial cells.
Koeppen K, Nymon A, Barnaby R, Li Z, Hampton TH, Ashare A, Stanton BA
Am J Physiol Lung Cell Mol Physiol. 2021 Apr 1;320(4):L530-L544. doi: 10.1152/ajplung.00621.2020. Epub 2021 Jan 20.
PMID: 33471607

Exposure to extracellular vesicles from Pseudomonas aeruginosa result in loss of DNA methylation at enhancer and DNase hypersensitive site regions in lung macrophages.
Kyung Lee M, Armstrong DA, Hazlett HF, Dessaint JA, Mellinger DL, Aridgides DS, Christensen BC, Ashare A
Epigenetics. 2020 Dec 31;:1-14. doi: 10.1080/15592294.2020.1853318. Epub 2020 Dec 31.
PMID: 33380271

Differential effects of the cystic fibrosis lung inflammatory environment on mesenchymal stromal cells.
Abreu SC, Hampton TH, Hoffman E, Dearborn J, Ashare A, Singh Sidhu K, Matthews DE, McKenna DH, Amiel E, Barua J, Krasnodembskaya A, English K, Mahon B, Dos Santos C, Cruz FF, Chambers DC, Liu KD, Matthay MA, Cramer RA, Stanton BA, Rocco PRM, Wargo MJ, Weiss DJ, Rolandsson Enes S
Am J Physiol Lung Cell Mol Physiol. 2020 Dec 1;319(6):L908-L925. doi: 10.1152/ajplung.00218.2020. Epub 2020 Sep 9.
PMID: 32901521

Extracellular Vesicles from Pseudomonas aeruginosa Suppress MHC-Related Molecules in Human Lung Macrophages.
Armstrong DA, Lee MK, Hazlett HF, Dessaint JA, Mellinger DL, Aridgides DS, Hendricks GM, Abdalla MAK, Christensen BC, Ashare A
Immunohorizons. 2020 Aug 20;4(8):508-519. doi: 10.4049/immunohorizons.2000026. Epub 2020 Aug 20.
PMID: 32819967

SARS-CoV-2 (COVID-19) and cystic fibrosis.
Stanton BA, Hampton TH, Ashare A
Am J Physiol Lung Cell Mol Physiol. 2020 Sep 1;319(3):L408-L415. doi: 10.1152/ajplung.00225.2020. Epub 2020 Jul 15.
PMID: 32668165

View more publications on PubMed