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George A. O'Toole Jr, PhD

Professor of Microbiology and Immunology

Microbiology and Immunology

University of Wisconsin - Madison, Ph.D., 1994
Cornell University, B.S., 1988

After postdoctoral work at the University of Wisconsin-Madison and Harvard Medical School, Dr. O'Toole joined the faculty of the Department of Microbiology at Dartmouth Medical School in 1999.

Molecular and Cellular Biology Graduate Programs

O'Toole Lab
Microbiology and Molecular Pathogenesis Program
Dept of Microbiology & Immunology
Molecular Cellular Biology Grad Program

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Contact Information

Geisel School of Medicine
Remsen Building, Rm 202 - HB 7550
Hanover NH 03755

Office: 202 Remsen
Phone: 603-650-1248
Email: georgeo@Dartmouth.Edu

Professional Interests

The main focus of the O'Toole laboratory is the study of complex surface-attached bacterial communities known as biofilms. Biofilms can form on a wide variety of surfaces including catheter lines, surgical implants, contact lenses, the lungs of patients with cystic fibrosis, industrial and drinking water pipelines, and on the surfaces of plant roots. In most natural, clinical, and industrial settings bacteria live predominantly in biofilms and not as planktonic (free-swimming) cells such as those typically studied in the laboratory. Bacteria growing in biofilm communities are of great interest to the medical community, because these bacteria become highly resistant to antibiotics by an as yet unknown mechanism. Although much has been learned about the types of microbes that can form biofilms, the morphology of these communities, and their chemical/physical properties, until recently little was known about the molecular genetic basis of biofilm formation or antibiotic resistance.

Studies in the O'Toole lab focus on:
>Polymicrobial infections and antibiotic tolerance in cystic fibrosis.
>The role of gut microbiota in airway disease in infants with cystic fibrosis.
>The signal transduction pathways regulating biofilm formation and surface sensing.
>The role of the intracellular signaling molecule c-di-GMP in controlling biofilm formation by Pseudomonads.

Selected Publications


Transcriptional profiling and genetic analysis of a cystic fibrosis airway-relevant model shows asymmetric responses to growth in a polymicrobial community.
Kesthely CA, Rogers RR, El Hafi B, Jean-Pierre F, O'Toole GA
Microbiol Spectr. 2023 Sep 29;:e0220123. doi: 10.1128/spectrum.02201-23. Epub 2023 Sep 29.
PMID: 37772884

Double-anonymous peer review comes to JB.
O'Toole GA
J Bacteriol. 2023 Sep 25;:e0008022. doi: 10.1128/jb.00080-22. Epub 2023 Sep 25.
PMID: 37747191

The regulator FleQ both transcriptionally and post-transcriptionally regulates the level of RTX adhesins of Pseudomonas fluorescens.
Pastora AB, O'Toole GA
J Bacteriol. 2023 Sep 26;205(9):e0015223. doi: 10.1128/jb.00152-23. Epub 2023 Sep 1.
PMID: 37655913

An In Vitro Medium for Modeling Gut Dysbiosis Associated with Cystic Fibrosis.
Barrack KE, Hampton TH, Valls RA, Surve SV, Gardner TB, Sanville JL, Madan JC, O'Toole GA
bioRxiv. 2023 Aug 2; pii: 2023.08.01.551570. doi: 10.1101/2023.08.01.551570. Epub 2023 Aug 2.
PMID: 37577487

Assessment of the Glycan-Binding Profile of Pseudomonas aeruginosa PAO1.
Sanchez H, O'Toole GA, Berwin B
Microbiol Spectr. 2023 Aug 17;11(4):e0166723. doi: 10.1128/spectrum.01667-23. Epub 2023 Jul 20.
PMID: 37470715

Development of the intestinal microbiome in cystic fibrosis in early life.
Price CE, Hampton TH, Valls RA, Barrack KE, O'Toole GA, Madan JC, Coker MO
mSphere. 2023 Aug 24;8(4):e0004623. doi: 10.1128/msphere.00046-23. Epub 2023 Jul 5.
PMID: 37404016

Evidence for the Type IV Pilus Retraction Motor PilT as a Component of the Surface Sensing System in Pseudomonas aeruginosa.
Geiger CJ, O'Toole GA
J Bacteriol. 2023 Jul 25;205(7):e0017923. doi: 10.1128/jb.00179-23. Epub 2023 Jun 29.
PMID: 37382531

Transcriptional Profiling and Genetic Analysis of a Cystic Fibrosis Airway-Relevant Model Shows Asymmetric Responses to Growth in a Polymicrobial Community.
Kesthely CA, Rogers RR, Hafi BE, Jean-Pierre F, O'Toole GA
bioRxiv. 2023 May 24; pii: 2023.05.24.542191. doi: 10.1101/2023.05.24.542191. Epub 2023 May 24.
PMID: 37293107

The Regulator FleQ Post-Transcriptionally Regulates the Production of RTX Adhesins by Pseudomonas fluorescens.
Pastora AB, O'Toole GA
bioRxiv. 2023 May 9; pii: 2023.05.09.540025. doi: 10.1101/2023.05.09.540025. Epub 2023 May 9.
PMID: 37214974

Evidence for the Type IV Pili Retraction Motor PilT as a Component of the Surface Sensing System in Pseudomonas aeruginosa.
Geiger CJ, O'Toole GA
bioRxiv. 2023 May 2; pii: 2023.05.02.539127. doi: 10.1101/2023.05.02.539127. Epub 2023 May 2.
PMID: 37205505

View more publications on PubMed