{"id":489,"date":"2017-05-02T16:04:45","date_gmt":"2017-05-02T16:04:45","guid":{"rendered":"https:\/\/geiselmed2.dartmouth.edu\/dartlab\/10-color-gallios-342w-borwell\/"},"modified":"2019-05-24T12:58:20","modified_gmt":"2019-05-24T16:58:20","slug":"10-color-gallios-342w-borwell","status":"publish","type":"page","link":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/instruments\/flow-cytometers\/10-color-gallios-342w-borwell\/","title":{"rendered":"10-color Gallios (710 Rubin)"},"content":{"rendered":"<p>Before you decide on a fluorochrome for a flow cytometric assay, you need to know:<\/p>\n<ul>\n<li>the wavelengths of the lasers in the cytometer<\/li>\n<li>the wavelengths of the filters in front of the photomultiplier tubes (PMTs) in the cytometer<\/li>\n<li>the absorption and fluorescence wavelengths of fluorochromes to be used<\/li>\n<\/ul>\n<p>These can be simulated using the <a title=\"BD fluorescence spectrum viewer\" href=\"http:\/\/m.bdbiosciences.com\/us\/s\/spectrumviewer\">BD fluorescence spectrum viewer<\/a><\/p>\n<p><span style=\"font-size: 14pt\"><strong><strong>Gallios Optical Bench<\/strong><\/strong><\/span><\/p>\n<p><strong> Violet laser configuration:<\/strong><\/p>\n<p><strong><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2017\/05\/Gallios-violet-April-2016.jpg\" alt=\"\" width=\"640\" height=\"360\" \/><br \/>\n<\/strong><strong style=\"font-size: 13px\">Blue laser configuration:<\/strong><\/p>\n<p><strong><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2017\/05\/Gallios-blue-Feb-2017.jpg\" alt=\"\" width=\"640\" height=\"360\" \/><br \/>\n<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2017\/05\/Gallios-blue-fluors.jpg\" alt=\"\" width=\"650\" height=\"291\" \/><\/p>\n<p><strong style=\"font-size: 13px\">Red laser configuration:<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Gallios red laser filters\" src=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2017\/05\/Gallios-red-April-2016.jpg\" alt=\"Gallios red laser filters\" width=\"640\" height=\"360\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2017\/05\/Gallios.red_.jpg\" alt=\"\" width=\"650\" height=\"278\" \/><\/p>\n<p>Gallios <a title=\"Gallios video from Beckman Coulter\" href=\"https:\/\/www.youtube.com\/watch?v=5TtOpfYwqoQ\">video<\/a> from Beckman Coulter<\/p>\n<p><strong>Stain index<\/strong><\/p>\n<p><strong><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2017\/05\/Gallios.jpg\" alt=\"\" width=\"650\" height=\"890\" \/><\/strong><\/p>\n<h2><strong>Click\u00a0<a title=\"Gallios Specifications\" href=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2017\/05\/Gallios_specs.pdf\" target=\"_blank\" rel=\"noopener\">here<\/a> for Gallios Specifications.<\/strong><\/h2>\n","protected":false},"excerpt":{"rendered":"<p>Before you decide on a fluorochrome for a flow cytometric assay, you need to know: the wavelengths of the lasers in the cytometer the wavelengths of the filters in front of the photomultiplier tubes (PMTs) in the cytometer the absorption and fluorescence wavelengths of fluorochromes to be used These can [\u2026] <\/p>\n<div class=\"clear\"><\/div>\n<p><a class=\"more_link clearfix\" href=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/instruments\/flow-cytometers\/10-color-gallios-342w-borwell\/\" rel=\"nofollow\">Read More<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":395,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-489","page","type-page","status-publish","hentry","author-2"],"jetpack_shortlink":"https:\/\/wp.me\/P9HkEC-7T","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/489","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/comments?post=489"}],"version-history":[{"count":2,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/489\/revisions"}],"predecessor-version":[{"id":1056,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/489\/revisions\/1056"}],"up":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/395"}],"wp:attachment":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/media?parent=489"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}