{"id":473,"date":"2017-05-02T16:04:45","date_gmt":"2017-05-02T16:04:45","guid":{"rendered":"https:\/\/geiselmed2.dartmouth.edu\/dartlab\/facsaria-ii-cell-sorter-336w-borwell\/"},"modified":"2023-10-27T11:23:14","modified_gmt":"2023-10-27T15:23:14","slug":"facsaria-ii-cell-sorter-336w-borwell","status":"publish","type":"page","link":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/instruments\/cell-sorters\/facsaria-ii-cell-sorter-336w-borwell\/","title":{"rendered":"FACSAria Fusion Cell Sorter (710 Rubin)"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-1331\" src=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Aria-Fusion-Banner-300x199.png\" alt=\"\" width=\"300\" height=\"199\" srcset=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Aria-Fusion-Banner-300x199.png 300w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Aria-Fusion-Banner-1024x680.png 1024w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Aria-Fusion-Banner-768x510.png 768w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Aria-Fusion-Banner-83x55.png 83w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Aria-Fusion-Banner-800x531.png 800w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Aria-Fusion-Banner-580x385.png 580w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Aria-Fusion-Banner.png 1142w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<p><span style=\"font-size: 18pt\"><strong>Before You Sort<\/strong><\/span><\/p>\n<p>All cell sorting experiments on the Fusion are operator-assisted. Before getting started with sorting, you will need to fill out the following online pre-sort questionnaire:<\/p>\n<p><a href=\"https:\/\/redcap.hitchcock.org\/redcap\/surveys\/?s=N4R83XFMLE\" target=\"_blank\" rel=\"noopener\">Cell Sorting Request Form<\/a><\/p>\n<p>To schedule a sort, please book the time of your sort on RaDar and also send an email to Gary Ward (gary.ward@dartmouth.edu).<\/p>\n<p>Staining panels for cell sorting must be optimized to allow for clear discrimination of populations of interest. The following optical bench information is necessary for designing a panel for a FACS experiment. We also recommend that you consult with DartLab staff prior to staining your sample for any pilot sort.<\/p>\n<p><span style=\"font-size: 18pt\"><strong>Optical Bench <\/strong><\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1332 size-full\" src=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Fusion-Optical-Bench.png\" alt=\"\" width=\"1952\" height=\"1733\" srcset=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Fusion-Optical-Bench.png 1952w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Fusion-Optical-Bench-300x266.png 300w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Fusion-Optical-Bench-1024x909.png 1024w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Fusion-Optical-Bench-768x682.png 768w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Fusion-Optical-Bench-62x55.png 62w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Fusion-Optical-Bench-1536x1364.png 1536w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Fusion-Optical-Bench-1600x1420.png 1600w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Fusion-Optical-Bench-800x710.png 800w, https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2023\/10\/Fusion-Optical-Bench-580x515.png 580w\" sizes=\"auto, (max-width: 1952px) 100vw, 1952px\" \/><\/p>\n<p><span style=\"font-size: 18pt\"><strong>Sorting Recovery Calculations<\/strong><\/span><\/p>\n<p>The following spreadsheet can be used to calculate cell recovery following an Aria sort:<\/p>\n<p><a href=\"https:\/\/geiselmed2.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/26\/2017\/05\/sortrecovery.xls\">Excel Spreadsheet Template for Sort Recovery Calculations<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Before You Sort All cell sorting experiments on the Fusion are operator-assisted. Before getting started with sorting, you will need to fill out the following online pre-sort questionnaire: Cell Sorting Request Form To schedule a sort, please book the time of your sort on RaDar and also send an email [\u2026] <\/p>\n<div class=\"clear\"><\/div>\n<p><a class=\"more_link clearfix\" href=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/instruments\/cell-sorters\/facsaria-ii-cell-sorter-336w-borwell\/\" rel=\"nofollow\">Read More<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":1121,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-473","page","type-page","status-publish","hentry","author-2"],"jetpack_shortlink":"https:\/\/wp.me\/P9HkEC-7D","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/473","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/comments?post=473"}],"version-history":[{"count":7,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/473\/revisions"}],"predecessor-version":[{"id":1334,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/473\/revisions\/1334"}],"up":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/1121"}],"wp:attachment":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/media?parent=473"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}