{"id":460,"date":"2017-05-02T14:04:50","date_gmt":"2017-05-02T18:04:50","guid":{"rendered":"https:\/\/geiselmed2.dartmouth.edu\/dartlab\/antibody-titration\/"},"modified":"2017-05-02T14:04:50","modified_gmt":"2017-05-02T18:04:50","slug":"antibody-titration","status":"publish","type":"page","link":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/services\/flow-cytometry\/antibody-titration\/","title":{"rendered":"Antibody Titration"},"content":{"rendered":"\n<p>An excellent discussion of antibody titration by John Altman (Emory Vaccine Center) may be found <a title=\"Altman ab titration\" href=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2017\/05\/Titering-Antibodies-Altman-Lab.pdf\">here<\/a>.<\/p>\n<p>\u00a0<\/p>\n<p>In addition, we suggest a plot of signal\/noise:<\/p>\n<p>\u00a0<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2017\/05\/titration.jpg\" alt=\"\" \/><\/p>\n<p>\u00a0<\/p>\n<p>Titer is affected by:\u2013Staining volume (e.g., 100 ul)\u2013Number of cells (not critical up to ~5 million cells)\u2013Staining time and temperature (e.g., 30 min RT)\u2013Type of sample (whole blood, PBMC, etc.)(Holden Maecker, Flow Cytometry Consulting)             <\/p>\n","protected":false},"excerpt":{"rendered":"<p>An excellent discussion of antibody titration by John Altman (Emory Vaccine Center) may be found here. \u00a0 In addition, we suggest a plot of signal\/noise: \u00a0 \u00a0 Titer is affected by:\u2013Staining volume (e.g., 100 ul)\u2013Number of cells (not critical up to ~5 million cells)\u2013Staining time and temperature (e.g., 30 min [\u2026] <\/p>\n<div class=\"clear\"><\/div>\n<p><a class=\"more_link clearfix\" href=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/services\/flow-cytometry\/antibody-titration\/\" rel=\"nofollow\">Read More<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":493,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-460","page","type-page","status-publish","hentry","author-2"],"jetpack_shortlink":"https:\/\/wp.me\/P9HkEC-7q","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/460","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/comments?post=460"}],"version-history":[{"count":0,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/460\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/493"}],"wp:attachment":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/media?parent=460"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}