Cell Preparation:<\/p>\n
http:\/\/media.ebioscience.com\/data\/pdf\/best-protocols\/cell-preparation-for-flow-cytometry.pdf<\/a><\/p>\n Annexin V:http:\/\/media.ebioscience.com\/data\/pdf\/best-protocols\/annexin-v-staining.pdf<\/a>(This shows with PI\/7-AAF and fixable viability dyes)Viability Staining:http:\/\/media.ebioscience.com\/data\/pdf\/best-protocols\/viability-staining.pdf<\/a>(Includes PI\/7-AAD and Fixable Dyes. eBioscience Specific)Cell surface staining:http:\/\/media.ebioscience.com\/data\/pdf\/best-protocols\/staining-cell-surface-antigens-for-flow-cytometry.pdf<\/a>Intracellular and Intranuclear Staining:http:\/\/media.ebioscience.com\/data\/pdf\/best-protocols\/staining-intracellular-antigens-for-flow-cytometry.pdf<\/a>Proliferation:http:\/\/media.ebioscience.com\/data\/pdf\/best-protocols\/proliferatione-dyes-for-flow-cytometry.pdf<\/a>(covers a couple eFluor dyes as well as CFSE; should double check that the CFSE concentration is consistent with our SOP)<\/p>\n <\/a><\/p>\n Tissue Dissociation Guide:<\/a> <\/a><\/a>http:\/\/www.worthington-biochem.com\/tissuedissociation\/default.html<\/a><\/p>\n http:\/\/www.maeckerlab.typepad.com\/<\/a><\/p>\n http:\/\/www.isac-net.org\/<\/a><\/p>\n How many events are enough?<\/a><\/p>\n Minimum information about a flow cytometry experiment<\/a> (MiFlowCyt)<\/p>\n Minimal Information About T cell Assays<\/a> (MIATA)<\/p>\n MIATA checklist<\/a><\/p>\n MIATA Easy Reporting of Experiments<\/a><\/p>\n Macrophage activation and polarization: nomenclature and experimental guidelines<\/a><\/p>\n BD fluorescence spectrum viewer<\/a><\/p>\n Amine-reactive dyes for dead cell discrimination in fixed samples (Perfetto et al., 2010)<\/a><\/p>\n BD compensation multicolor technical bulletin (2009)<\/a><\/p>\n Optimizing a multi-color immunophenotyping assay (Mahnke & Roederer, 2007)<\/a><\/p>\n