{"id":425,"date":"2017-05-02T16:04:56","date_gmt":"2017-05-02T16:04:56","guid":{"rendered":"https:\/\/geiselmed2.dartmouth.edu\/dartlab\/elispot\/"},"modified":"2017-05-02T16:04:56","modified_gmt":"2017-05-02T16:04:56","slug":"elispot","status":"publish","type":"page","link":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/immunoassays\/elispot\/","title":{"rendered":"ELISPOT"},"content":{"rendered":"\n<p>The Enzyme-linked Immunospot (ELISPOT) assay is a method that allows the determination of T or B cell responses by allowing enumeration of cells secreting a specific product (cytokine or antibody). As an example, for a T cell ELISPOT, a PVDF plate is coated with antibody towards a particular cytokine. Cells are then incubated with antigen or mitogen, secreting cytokine that is bound by the antibody on the plate. The cells are washed away, and a biotinylated detection antibody specific for the cytokine is added. Enzyme-conjugated streptavidin and substrate is then added, revealing a spot on the membrane for each cytokine-secreting cell. The ELISPOT assay is 10 times more sensitive than flow cytometry whe trying to enumerate antigen-specific cells.<\/p>\n<p><a href=\"https:\/\/en.wikipedia.org\/wiki\/ELISPOT#\/media\/File:ELISPOT-en.png\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-content\/uploads\/sites\/22\/2017\/05\/ELISPOT-en.png\" alt=\"\" width=\"517\" height=\"404\" \/><\/a><\/p>\n<p>For a generalized ELISPOT protocol, click <a title=\"ELISPOT protocol\" href=\"http:\/\/zellnet.com\/services\/protocols\/general-elispot-protocol\/\">here<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Enzyme-linked Immunospot (ELISPOT) assay is a method that allows the determination of T or B cell responses by allowing enumeration of cells secreting a specific product (cytokine or antibody). As an example, for a T cell ELISPOT, a PVDF plate is coated with antibody towards a particular cytokine. Cells [\u2026] <\/p>\n<div class=\"clear\"><\/div>\n<p><a class=\"more_link clearfix\" href=\"https:\/\/geiselmed.dartmouth.edu\/dartlab\/immunoassays\/elispot\/\" rel=\"nofollow\">Read More<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":488,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-425","page","type-page","status-publish","hentry","author-2"],"jetpack_shortlink":"https:\/\/wp.me\/P9HkEC-6R","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/425","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/comments?post=425"}],"version-history":[{"count":0,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/425\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/488"}],"wp:attachment":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/media?parent=425"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}