{"id":1114,"date":"2020-02-14T15:44:44","date_gmt":"2020-02-14T20:44:44","guid":{"rendered":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/?page_id=1114"},"modified":"2025-07-23T14:54:48","modified_gmt":"2025-07-23T18:54:48","slug":"cytoflex","status":"publish","type":"page","link":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/instruments\/flow-cytometers\/cytoflex\/","title":{"rendered":"CytoFLEX (2 Remsen)"},"content":{"rendered":"

The CytoFLEX<\/strong> (Beckman Coulter) is a four laser, 15 detector analysis flow cytometer capable of measuring 13 colors<\/strong> simultaneously. The lasers in this instrument are Violet (405nm), Blue (488nm), Yellow-Green (561nm),\u00a0and Red (638nm). \u00a0The Wavelength Division Multiplexer\u00a0(WDM) deconstructs and measures multiple wavelengths of light,\u00a0relying on fiber optics and band pass filters to separate the light\u00a0wavelengths.\u00a0The WDM utilizes Avalanche photodiode detectors versus\u00a0photomultiplier tubes, resulting in a\u00a0high quantum efficiency in excess of 80%, especially for\u00a0wavelengths greater than 800 nm. \u00a0The CytoFLEX also has a high throughput 96 well plate loader.<\/p>\n

 <\/p>\n\n\n\n\n\n\n
Location
\n(BSL2)<\/td>\n		Remsen, room 243C<\/td>\nBook the CytoFLEX<\/a><\/span><\/td>\n<\/tr>\n
Rates<\/td>\n\u00a0Dartmouth: Self-operated $50 per hour
\nPharma: Self-operated $160 per hour\/Assisted $180 per hour plus $180 set-up feeBilling policy: Users are billed for the \u00a0greater of time reserved or actual time used on the instrument.
\nCancellation policy: Users must cancel their reservations 24 hours in advance of their scheduled appointment to avoid \u00a0charges.<\/td>\n<\/tr>\n
Hours<\/td>\n\u00a024 hours \/ 7 days a week<\/td>\n<\/tr>\n
PMTs<\/td>\n\u00a0Violet: 450\/45, 525\/40, 610\/20, 660\/20<\/p>\n

Blue: SSC, 525\/40, 690\/50<\/p>\n

Yellow: 561\/10, 585\/42, 610\/20 (mCherry), 690\/50, 780\/60<\/p>\n

Red: 660\/20, 712\/25, 780\/60<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Online training:<\/strong><\/p>\n

Basic Use: https:\/\/youtu.be\/y-IktIXcM2U?si=2yCxBBLMksc-Pvab<\/a><\/p>\n

Video for small particle detection: \u201cExtracellular vesicle detection and analysis via flow cytometry\u201d, Vasilis Toxavidis and John Tigges:\u00a0https:\/\/www.youtube.com\/watch?v=csnPPY-8FZk<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

The CytoFLEX (Beckman Coulter) is a four laser, 15 detector analysis flow cytometer capable of measuring 13 colors simultaneously. The lasers in this instrument are Violet (405nm), Blue (488nm), Yellow-Green (561nm),\u00a0and Red (638nm). \u00a0The Wavelength Division Multiplexer\u00a0(WDM) deconstructs and measures multiple wavelengths of light,\u00a0relying on fiber optics and band pass [\u2026] <\/p>\n

<\/div>\n

Read More<\/a><\/p>\n","protected":false},"author":42,"featured_media":0,"parent":395,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1114","page","type-page","status-publish","hentry","author-42"],"jetpack_shortlink":"https:\/\/wp.me\/P9HkEC-hY","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/1114","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/users\/42"}],"replies":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/comments?post=1114"}],"version-history":[{"count":33,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/1114\/revisions"}],"predecessor-version":[{"id":1413,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/1114\/revisions\/1413"}],"up":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/pages\/395"}],"wp:attachment":[{"href":"https:\/\/geiselmed.dartmouth.edu\/dartlab\/wp-json\/wp\/v2\/media?parent=1114"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}