{"id":36,"date":"2017-12-01T20:13:03","date_gmt":"2017-12-01T20:13:03","guid":{"rendered":"https:\/\/geiselmed2.dartmouth.edu\/compton\/?page_id=36"},"modified":"2017-12-14T18:41:28","modified_gmt":"2017-12-14T18:41:28","slug":"photos","status":"publish","type":"page","link":"https:\/\/geiselmed.dartmouth.edu\/compton\/photos\/photos\/","title":{"rendered":"Lab Photos"},"content":{"rendered":"<p class=\"smalltext\">(for details, click on each image)<\/p>\n<div id=\"gallery-1\" class=\"gallery galleryid-36 gallery-columns-3 gallery-size-thumbnail\">\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Kif2a.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Localization of the KinI kinesin Kif2a (red) in a human cell during metaphase.  Chromosomes are stained blue.  See Ganem and Compton, 2004.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Kif2a-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-686\">Kif2a<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/disrupted_spindle_pole.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Inhibition of NuMA and HSET disrupt microtubule minus end focusing at spindle poles. Also, the centrosome-nucleated asters are no longer tethered to other spindle microtubules. Microtubules are stained green and chromosomes are stained blue. See Gordon et al., 2001.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/disrupted_spindle_pole-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-681\">Disrupted spindle poles<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/HSET.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Localization of the kinesin HSET (red) in a human cell during metaphase. Chromosomes are stained blue. See Mountain et al, 1999.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/HSET-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-682\">HSET<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/kinetocher_fibers.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"The stable kinetochore microtubules (green) are revealed when calcium is used to deploymerize all other spindle microtubules that are less stable. Chromosomes are stained blue. See Levesque et al., 2003.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/kinetocher_fibers-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-687\">Kinetochore Fibers<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Mitotic_spindle.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Metaphase spindle organization in a human cell. Microtubules are stained green, centrosomes are stained red and chromosomes are stained blue. See Levesque et al., 2003\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Mitotic_spindle-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-692\">Mitotic Spindle<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Monopolar.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Bipolar spindle assembly fails following inhibition of the KinI kinesin Kif2a. Microtubules are stained green and chromosomes are stained blue. See Ganem and Compton, 2004.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Monopolar-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-693\">Monopolar Spindle<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/NuMA.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Localization of the structural protein NuMA (red) in a human cell during metaphase. Chromosomes are stained blue.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/NuMA-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-694\">NuMA<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/interphase_microtubules.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Taxol-induced polymerization of microtubules in extracts prepared from interphase cells. See Gaglio et al., 1995.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/interphase_microtubules-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-684\">Microtubules in Interphase Extracts<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/microtubule_asters.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Taxol-induced polymerization of microtubules in extracts prepared from mitotic cells. Note the organization of microtubules in radial arrays or microtubule asters. See Gaglio et al., 1995.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/microtubule_asters-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-691\">Microtubules in Mitotic Extracts<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon portrait\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/spindle_pole_electron_micro.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Transmission electron micrograph of the spindle pole in a PtK2 cell. The centrioles are visible and NuMA has been labeled with small gold particles (very tiny black dots). See Dionne et al., 1999.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/spindle_pole_electron_micro-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-698\">Spindle Pole Viewed by TEM<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/astrin.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Localization of the the spindle protein astrin (red) in a human cell during metaphase. Chromosomes are stained blue. See Mack and Compton, 2001.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/astrin-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-679\">Astrin<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/computer_simulated_aster.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Organization of microtubules into astral arrays by the actions of motors and non-motor cross linking proteins. This is the output from the computer simulation of these activities. See Chakravarty et al., 2003.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/computer_simulated_aster-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-680\">Simulated microtubule aster<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/LaggingChromatid.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Perturbation of the function of the kinesin-13 proteins MCAK and Kif2a leads to high frequencies of lagging chromatids at anaphase.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/LaggingChromatid-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-688\">Lagging Chromatids at Anaphase<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/U2OS_DIC.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Perturbation of the function of the kinesin-13 proteins MCAK and Kif2a eliminates polewards microtubule flux, but does not interfere with chromosome alignment as shown in this DIC image.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/U2OS_DIC-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-700\">Chromosome Alignment in Mitosis<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/TSC1.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Phospho-S6 staining in TSC1-\/- cells\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/TSC1-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-699\">Phospho-S6 staining in TSC1-\/- cells<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Spindle_MT_Kif2b.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Spindle MT Kif2b\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Spindle_MT_Kif2b-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-697\">Spindle MT Kif2b<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon portrait\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/human_diploid.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Fluorescence in situ hybridization of human diploid HCT116 cells using centromere-specific probes for chromosomes 7 (green) and 8 (red).\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/human_diploid-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-683\">Human diploid HCT116 cells<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon landscape\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/phase_contrast_asters.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Phase contrast microscopy reveals the differences between microtubule asters formed in a mammalian mitotic extract with and without the motor protein Eg5.\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/phase_contrast_asters-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-695\">Microtubule Asters<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon portrait\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Merotely_metaphase.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Merotely Metaphase\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Merotely_metaphase-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-689\">Merotely Metaphase<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon portrait\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/metaphase_warhol.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Metaphase Warhol\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/metaphase_warhol-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-690\">Metaphase Warhol<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon portrait\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/RPE1_Kabeche.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"RPE1 Kabeche\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/RPE1_Kabeche-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-696\">RPE1 Kabeche<\/figcaption><\/figure>\n<figure class=\"gallery-item\">\n<div class=\"gallery-icon portrait\">\n      <a href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Kabeche_Cover4.jpg\" class=\"thickbox\" rel=\"gallery\" title=\"Kabeche Cover\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" src=\"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-content\/uploads\/sites\/30\/2017\/12\/Kabeche_Cover4-150x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\"><\/a>\n    <\/div><figcaption class=\"wp-caption-text gallery-caption\" id=\"gallery-1-685\">Kabeche Cover<\/figcaption><\/figure>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>(for details, click on each image) Kif2a Disrupted spindle poles HSET Kinetochore Fibers Mitotic Spindle Monopolar Spindle NuMA Microtubules in Interphase Extracts Microtubules in Mitotic Extracts Spindle Pole Viewed by TEM Astrin Simulated microtubule aster Lagging Chromatids at Anaphase Chromosome Alignment in Mitosis Phospho-S6 staining in TSC1-\/- cells Spindle MT [\u2026] <\/p>\n<div class=\"clear\"><\/div>\n<p><a class=\"more_link clearfix\" href=\"https:\/\/geiselmed.dartmouth.edu\/compton\/photos\/photos\/\" rel=\"nofollow\">Read More<\/a><\/p>\n","protected":false},"author":36,"featured_media":0,"parent":24,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-36","page","type-page","status-publish","hentry","author-36"],"_links":{"self":[{"href":"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-json\/wp\/v2\/pages\/36","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-json\/wp\/v2\/users\/36"}],"replies":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-json\/wp\/v2\/comments?post=36"}],"version-history":[{"count":1,"href":"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-json\/wp\/v2\/pages\/36\/revisions"}],"predecessor-version":[{"id":897,"href":"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-json\/wp\/v2\/pages\/36\/revisions\/897"}],"up":[{"embeddable":true,"href":"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-json\/wp\/v2\/pages\/24"}],"wp:attachment":[{"href":"https:\/\/geiselmed.dartmouth.edu\/compton\/wp-json\/wp\/v2\/media?parent=36"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}