Principal Investigator, Professor of Molecular and Systems Biology
Dr. Whitfield is a Professor in the Department of Molecular and Systems Biology at the Geisel School of Medicine. He graduated with honors from North Carolina State University with degrees in biochemistry and chemistry. He received his PhD from University of North Carolina, Chapel Hill in Biochemistry and Biophysics working with Dr. William Marzluff and then performed post-doctoral training with Drs. David Botstein and Patrick Brown at Stanford University School of Medicine. Dr. Whitfield was awarded the 2002 Paper of the Year by the American Society for Cell Biology (Whitfield et al. 2002 MBC), he was a V Scholar for Cancer Research, was named a Hulda Irene Duggan Arthritis Investigator, and is the recipient of multiple NIH grants. When not in lab, he can often been found racing his bike with Team HUP United.
B.S. Biochemistry, Molecular and Cellular Biology, University of New Hampshire
My current focus is on optimizing NanoString technology to investigate the gene expression profiles of samples from patients with SSc in order to classify SSc patients into intrinsic subsets.
Postdoctoral Fellow - Ph.D. from The University of Texas MD Anderson Cancer Center, Houston, TX
My current study is focusing on selecting predictive markers for SSc severity. Also, I am identifying the SSc severity-responsible microRNAs. In addition, I hope to develop RNA-seq differential expression (DE) analysis methods tailored to our SSc studies.
Jennifer M. Franks
Graduate Student - B.S. Applied Statistics, B.S. Genetics, Purdue University
I am currently working on utilizing intrinsic molecular subsets to understand disease trajectory and characterizing the immune repertoire in patients with SSc.
Postdoctoral Fellow - Ph.D. from Dartmouth College, Hanover, NH
SSc gene expression data analysis with emphasis on clinical trials.
Bhaven K. Mehta
Graduate Student - B.A. Biochemistry and Molecular Biology; M.A. Biotechnology, Boston University
Bhaven is performing RNA-sequencing on multiple organs (skin, esophagus, fundus, duodenum, and blood) from individual patients with SSc to assess whether the traditional molecular subsets found in SSc skin are conserved across multiple tissues of an individual. Bhaven is also performing RNA-sequencing on an additional cohort to evaluate the differences and similarities between the upper and lower esophagus of patients with SSc.
Graduate Student - B.S. Biochemistry, Stony Brook University
I am using computational tools to determine the recapitulation viability of SSc in three-dimensional tissue matrices and two-dimensional tissue matrices. I am also working on adapting computational methods to determine immune cell infiltrate within patient tissue samples, specifically macrophages.
Diana M. Toledo
Graduate Student - B.A. Biology, Brown University; M.S. Genetic Counseling, Boston University
I am currently investigating the molecular outcomes of patients with SSc during different time points throughout the mycophenolate mofetil (MMF) drug trial. In addition, I am working on identifying DNA methylation signatures in SSc subsets and SSc-causing cell types (like macrophages).
Tammara A. Wood
Project Coordinator - Master of Science
Tammara is the project coordinator for NIAMS Scleroderma Center Of Research Translation; National Scleroderma Core Centers investigating the genome-wide gene expression profiles of the autoimmune disease systemic sclerosis (SSc).
Lee is utilizing deep sequencing technology to define cis-elements that play crucial roles in RNA biology.
Lab technician - B.A. from Dartmouth College
I am studying the cell cycle-regulated gene Fam64A, whose knockdown leads to monopolar spindles, and characterizing its interactions with members of the chromosomal passenger complex and positives from a yeast two-hybrid screen.
Lacy is originally from Atlanta, GA, and earned her B.S. in Biology cum laude from Mercer University in Macon, GA. She is currently working on a previously unannotated cell cycle-regulated gene, Fam64A. Using microarray data of hierarchically clustered cell cycle-regulated genes, she hypothesized that the protein is involved in spindle assembly, and indeed found that knockdown of Fam64A with siRNA leads to an increase in monopolar spindles. She is currently working to further characterize the function of Fam64A and determine the mechanisms that lead to this spindle defect. She is also working to identify cell cycle-regulated genes in an untransformed cell line to investigate cell type-specific differences in cell cycle-regulated genes.
Postdoc - Ph.D.
Gavin studies how forkhead box genes regulate cell cycle gene expression and the cell cycle regulated genes in U2OS cells.
Michael E. Johnson
Postdoctoral Fellow - Ph.D. from Drexel University College of Medicine, Philadelphia, PA
I am currently investigating the role various signaling pathways play in driving pathogenesis of scleroderma. Additionally, I am investigating the role of enhanced gene expression in SSc fibroblasts.
Zhenghui "Jerry" Li
Graduate Student - B.S. Microbiology, cum laude
Jerry received his B.S. in Microbiology (with honor) from University of Rochester in 2010. While in Rochester, Jerry worked on the anti-viral immune response in frog Xenopus laevis under the instruction of Dr. Jacques Robert. Here in Whitfield Lab, Jerry works on analyzing RNA-Seq data from human scleroderma tissue. In addition, Jerry works on correlating different mice models to human scleroderma sub types by microarray.
Postdoctoral Fellow - Ph.D.
Meta-analysis of scleroderma gene expression microarray data.
Sarah came to the Whitfield lab by way of the Thayer School of Engineering at Dartmouth, where she received an M.S. in Biomedical Engineering. At Thayer School, in the labs of Dr. Paul Meaney and Dr. Marvin Doyley, she researched alternative breast cancer imaging modalities as well as alternative atherosclerosis imaging methods. Here, in the Whitfield Lab, Sarah is using microarray technology to investigate the genome-wide gene expression profiles of skin and blood samples from patients with the autoimmune disease systemic sclerosis (SSc) to identify biomarkers and to extend and validate SSc subsets. When Sarah is not working hard in the lab, she donates time to support local good-will causes such as the The Haven, a local shelter for homeless families.
Jenn is originally from Brisbane Australia where she received a BSc and Honors Class I in Biochemistry from the University of Queensland. Before coming to the Whitfield Lab, Jenn was a post-graduate researcher at UCSF studying signal transduction pathways in Pseudomonas aeruginosa. Jenn's research currently centers around the hypothesis that different mouse models may each resemble a different subtype of scleroderma disease. She has extensively characterized the gene expression signatures in murine cGVHD skin and found that gene expression bears a remarkable similarity to that found in the 'Inflammatory' subset of scleroderma patients. She is currently analyzing the TSK1 & TSK2 models and the bleomycin-induced fibrosis models to examine their similarities to human SSc in the context of the intrinsic subsets. She has also defined in vitro mechanistic signatures for profibrotic cytokines TGF, IL13 and IL4 in dermal fibroblasts and has identified differential expression of these signaling pathways in the intrinsic subsets found in scleroderma skin. Outside of lab Jenn is an avid foodie, road biker, climber and crossfitter, and in addition to being a graduate student is also a dedicated crazy cat lady.
Jaclyn N. Taroni
Graduate Student - B.S. Biology, Lehigh University
I work on functional genomic meta-analyses of multiple tissues and clinical manifestations of systemic sclerosis.