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Henry N. Higgs, Ph.D.

Title(s):
Professor of Biochemistry

Department(s):
Biochemistry

Education:
Lafayette College, BA 1987U. Washington, PHD 1996

Programs:
Molecular and Cellular Biology Graduate Programs

Contact Information:

Dartmouth Medical School
HB 7200
Hanover NH 03755


Professional Interests:

Microvilli cover the surface of circulating blood lymphocytes. Lymphocytes use these finger-like projections to segregate adhesion receptors, as certain receptors localize to microvilli while others are excluded from them. Adhesion receptor segregation is crucial because cells use these receptors sequentially when moving from the bloodsteam to specific tissues. Little is known about the architecture of microvilli, nor about their relationship to similar structures from other cells. My lab will identify protein components crucial to microvillar structure, test how mutation of these proteins affect microvillar structure and function, and characterize how these proteins function biochemically and biophysically.


Selected Publications:

 

Guo B, Gurel PS, Shu R, Higgs HN, Pellegrini M, Mierke DF
Monitoring ATP hydrolysis and ATPase inhibitor screening using (1)H NMR.
Chem Commun (Camb) 2014 Sep 16; 50(81):12037-9
PMID: 25170530

Gurel PS, Hatch AL, Higgs HN
Connecting the cytoskeleton to the endoplasmic reticulum and Golgi.
Curr Biol 2014 Jul 21; 24(14):R660-72
PMID: 25050967

Korobova F, Gauvin TJ, Higgs HN
A role for myosin II in mammalian mitochondrial fission.
Curr Biol 2014 Feb 17; 24(4):409-14
PMID: 24485837

Kim J, Shapiro MJ, Bamidele AO, Gurel P, Thapa P, Higgs HN, Hedin KE, Shapiro VS, Billadeau DD
Coactosin-like 1 antagonizes cofilin to promote lamellipodial protrusion at the immune synapse.
PLoS One 2014; 9(1):e85090
PMID: 24454796

Gurel PS, Ge P, Grintsevich EE, Shu R, Blanchoin L, Zhou ZH, Reisler E, Higgs HN
INF2-mediated severing through actin filament encirclement and disruption.
Curr Biol 2014 Jan 20; 24(2):156-64
PMID: 24412206

Ramabhadran V, Hatch AL, Higgs HN
Actin monomers activate inverted formin 2 by competing with its autoinhibitory interaction.
J Biol Chem 2013 Sep 13; 288(37):26847-55
PMID: 23921379

Korobova F, Ramabhadran V, Higgs HN
An actin-dependent step in mitochondrial fission mediated by the ER-associated formin INF2.
Science 2013 Jan 25; 339(6118):464-7
PMID: 23349293

Thompson ME, Heimsath EG, Gauvin TJ, Higgs HN, Kull FJ
FMNL3 FH2-actin structure gives insight into formin-mediated actin nucleation and elongation.
Nat Struct Mol Biol 2013 Jan; 20(1):111-8
PMID: 23222643

Ramabhadran V, Gurel PS, Higgs HN
Mutations to the formin homology 2 domain of INF2 protein have unexpected effects on actin polymerization and severing.
J Biol Chem 2012 Oct 5; 287(41):34234-45
PMID: 22879592

Heimsath EG Jr, Higgs HN
The C terminus of formin FMNL3 accelerates actin polymerization and contains a WH2 domain-like sequence that binds both monomers and filament barbed ends.
J Biol Chem 2012 Jan 27; 287(5):3087-98
PMID: 22094460