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Carmen J Marsit, Ph.D.

Title(s):
Associate Professor of Pharmacology & Toxicology
Associate Professor of Community and Family Medicine
Co-Director, Program in Cancer Epidemiology, Norris Cotton Cancer Center

Department(s):
Pharmacology & Toxicology
Community and Family Medicine

Education:
Harvard University, Cambridge, MA, Ph.D. 2004

Programs:
Norris Cotton Cancer Center
Program in Experimental and Molecular Medicine
Quantitative Biomedical Sciences

Websites:
http://geiselmed.dartmouth.edu/marsit/
http://geiselmed.dartmouth.edu/pharmtox/
http://cancer.dartmouth.edu/res/cancer_epi_chemo.html
http://www.dartmouth.edu/~childrenshealth/

Contact Information:

HB 7650
Pharmacology & Toxicology
Hanover NH 03755

Office: Remsen 520
Phone: 603-650-1825
Fax: 603-650-1129
Email: Carmen.J.Marsit@Dartmouth.edu


Professional Interests:

The broad goal of Dr. Marsitís research program is to understand how the environment contributes to health and disease by studying molecular mechanisms that may be responsible. The laboratory's focus is on epigenetic regulation and works to describe the impact the environment has on the human epigenome.
This work is accomplished by taking an interdisciplinary and multi-modal approach to understanding the pathogenesis of human disease, utilizing the power of epidemiology and population-based research to study the effects of the environment on multiple facets of epigenetic regulation while examining mechanistic questions in controlled in-vitro experiments. Dr. Marsitís research has examined environment epigenetic impacts on two distinct, yet highly related biologic processes: carcinogenesis and human development. In those settings, his laboratory examines DNA methylation and miRNA expression as key epigenetic mechanisms of interest.
This research aims to provide a sound scientific basis for the emerging paradigm linking the environment experienced during in utero development to health and disease throughout life.

Studies in the laboratory are aimed at identifying novel biomarkers of cancer risk and prognosis through the examination of epigenetic alterations. The laboratory uses genome-scale tools to examine DNA methylation and miRNA expression in human clinical samples, and through careful analysis, to identify novel markers, which we can then translate into clinical use.

Other studies examine the hypothesis that the intrauterine environment experienced by a developing fetus can influence the epigenome of the infantís placenta. This functional alteration of the placenta can thereby program the health of the infant both at birth and likely well beyond. Some of the earliest phenotypes which can be examined to test this hypothesis are those of neurobehavioral development. Through collaborations at Women and Infant Hospital of Rhode Island, the Childrenís Environmental Health and Disease Prevention Center at Dartmouth, and Mt. Sinai School of Medicine, Dr. Marsit and his laboratory are profiling the placental epigenome and identifying relationships between epigenetic mechanisms at work in the placenta and the infantís newborn neurobehavior.

Paramount to meeting the objectives of this research program is creating a collaborative and multidisciplinary team of students, clinicians, epidemiologists, biologists, and statisticians who, by working together, are committed to combining efforts to reach these goals. Such a combined effort and interdisciplinary training is absolutely necessary to accomplish this work, and will certainly open up entirely new avenues for research both here at Dartmouth and beyond.

Rotations and Thesis Projects:

1. Examine how the intrauterine environment during pregnancy alters DNA methylation in infant placenta and cord blood and the downstream consequences of these alterations
2. Identify and expand on novel epigenetic biomarkers for cancer risk and prognosis in bladder and skin cancers.
3. Examine alterations in genomic imprinting in human placenta and its association with environmental exposures, and newborn developmental outcomes.

Grant Information:

R01 MH094609 (Marsit) Epigenetics in Neurodevelopment and Mental Health (NIH/NIMH)

R01 ES022223 (Marsit and Chen) Environment, Imprinting, and Neurodevelopment (NIH/NIEHS)

P01 ES022832 (Karagas, Marsit and Robbins) Childrenís Environmental Health and Disease Prevention Center Project 3: Placental Biomarkers of Exposure and Outcome (NIH/NIEHS and US EPA)

Courses Taught:

PEMM 103: Introduction to Biostatistics

Biography:

Dr. Marsit received his B.S. from Lafayette College in Biochemistry in 2000, and his Ph.D. from the Program in Biological Sciences in Public Health at Harvard University in 2004. He trained as postdoctoral research associate in molecular epidemiology at the Harvard School of Public Health. In 2007, he was appointed as Assistant Professor in the Department of Pathology and Laboratory Medicine, at Brown University. He joined the faculty at Dartmouth in 2011, as an Assistant Professor in the Departments of Pharmacology and Toxicology and Community and Family Medicine in the Section of Biostatistics and Epidemiology.


Selected Publications:

 

Green BB, Kappil M, Lambertini L, Armstrong DA, Guerin DG, Sharp AJ, Lester BM, Chen J, Marsit CJ
Expression of imprinted genes in placenta is associated with infant neurobehavioral development.
Epigenetics 2015 Jul 22; :0
PMID: 26198301

Kappil MA, Green BB, Armstrong DA, Sharp AJ, Lambertini L, Marsit CJ, Chen J
Placental Expression Profile of Imprinted Genes Impacts Birth Weight.
Epigenetics 2015 Jul 17; :0
PMID: 26186239

Conradt E, Fei M, LaGasse L, Tronick E, Guerin D, Gorman D, Marsit CJ, Lester BM
Prenatal predictors of infant self-regulation: the contributions of placental DNA methylation of NR3C1 and neuroendocrine activity.
Front Behav Neurosci 2015; 9:130
PMID: 26074794

Marsit CJ, Brummel SS, Kacanek D, Seage GR 3rd, Spector SA, Armstrong DA, Lester BM, Rich K
Infant Peripheral Blood Repetitive Element Hypomethylation Associated with Antiretroviral Therapy in utero.
Epigenetics 2015 Jun 11; :0
PMID: 26067216

Cheng C, Varn FS, Marsit CJ
E2F4 Program is Predictive of Progression and Intravesical Immunotherapy Efficacy in Bladder Cancer.
Mol Cancer Res 2015 Jun 1;
PMID: 26032289

Tyrka AR, Parade SH, Eslinger NM, Marsit CJ, Lesseur C, Armstrong DA, Philip NS, Josefson B, Seifer R
Methylation of exons 1D, 1F, and 1H of the glucocorticoid receptor gene promoter and exposure to adversity in preschool-aged children.
Dev Psychopathol 2015 May; 27(2):577-85
PMID: 25997773

Maccani JZ, Koestler DC, Houseman EA, Armstrong DA, Marsit CJ, Kelsey KT
DNA methylation changes in the placenta are associated with fetal manganese exposure.
Reprod Toxicol 2015 May 15;
PMID: 25982381

Cardenas A, Koestler DC, Houseman EA, Jackson BP, Kile ML, Karagas MR, Marsit CJ
Differential DNA methylation in umbilical cord blood of infants exposed to mercury and arsenic in utero.
Epigenetics 2015; 10(6):508-15
PMID: 25923418

Houseman EA, Kelsey KT, Wiencke JK, Marsit CJ
Cell-composition effects in the analysis of DNA methylation array data: a mathematical perspective.
BMC Bioinformatics 2015 Mar 21; 16:95
PMID: 25887114

Liang C, Kelsey KT, McClean MD, Christensen BC, Marsit CJ, Karagas MR, Waterboer T, Pawlita M, Nelson HH
A coding variant in TMC8 (EVER2) is associated with high risk HPV infection and head and neck cancer risk.
PLoS One 2015; 10(4):e0123716
PMID: 25853559