Edit Entry

William R Green, Ph.D.

Title(s):
Department Chair
Chair and Professor of Microbiology and Immunology
The Elmer R. Pfefferkorn Professor and Chair of the Department of Microbiology and Immunology

Department(s):
Microbiology and Immunology

Education:
Case Western Reserve University, PHD 1977
University of Michigan, BS 1972

Dr. Green's subsequent postdoctoral work was supported by an individual NIH postdoctoral fellowship, and he was a research associate in Immunology at first Johns Hopkins University School of Medicine and then at the Fred Hutchinson Cancer Research Center (FHCRC) and the University of Washington (UW) in Seattle. He became an Assistant Member/Professor at FHCRC and the UW in 1980. Dr. Green joined the faculty of the Department of Microbiology at Dartmouth Medical School in 1983. From 1992-2002 Dr. Green served as Director of the Immunology Program, including the Immunology and Cancer Immunotherapy Program of the Norris Cotton Cancer Center, before becoming Chair of the Microbiology and Immunology (M/I) Department in July, 2002. From January, 2008 through September 2010, he served a one term as Dean of Dartmouth Medical School before returning to chairmanship of the M/I Department, and Director of the Dartmouth Community Medical School.

Programs:
Immunology Program
Molecular and Cellular Biology Graduate Programs
Norris Cotton Cancer Center
Program in Experimental and Molecular Medicine

Websites:
http://dms.dartmouth.edu/microbio/
http://dms.dartmouth.edu/immuno/
http://www.dartmouth.edu/~mcb/
http://dms.dartmouth.edu/COBRE/

Contact Information:

Dartmouth Medical School
Borwell Research Bldg., HB 7556
1 Medical Center Drive
Lebanon NH 03756

Email: William.R.Green@Dartmouth.EDU


Professional Interests:

T Cell Immune Responses to Retroviral Diseases

The primary interests of the lab focus on cell-mediated immunity to mouse retroviruses that cause either leukemia or immunodeficiency. We have been studying resistance to endogenous AKR/Gross virus-induced leukemia mediated by cytotoxic T lymphocyte (CTL) responses. By use of "low leukemia" mouse strains, CTL that readily lyse AKR/Gross retrovirus-induced tumors have been generated. The CTL recognize "type-specific" viral determinants with the major immunodominant viral peptide located in the transmembrane anchor protein encoded by the envelope gene. Other mouse strains of higher leukemia incidence are unable to generate such CTL responses. The mechanism of unresponsiveness include both MHC-dependent mechanisms and apoptosis of effector CTL following interaction with FasL-expressing virus infected cells.

Another area of emphasis is the study of both immunity to the mouse acquired immunodeficiency syndrome (MAIDS) retroviral isolate and the mechanism of retroviral pathogenesis. In this model of HIV induced AIDS, we have raised unique protective CTL to the causative virus. Interestingly, the immunodominant determinant recognized by the CTL is encoded by a previously unrecognized alternative viral translational reading frame. Moreover, this alternative reading frame is extended, and mutagenesis experiments have shown that it exists because its protein product is necessary for viral pathogenesis. We are also interested in novel vaccine approaches, particularly to the Category A biodefense agent, smallpox virus.

Biography:

Dr. Green's subsequent postdoctoral work was supported by an individual NIH postdoctoral fellowship, and he was a research associate in Immunology at first Johns Hopkins University School of Medicine and then at the Fred Hutchinson Cancer Research Center (FHCRC) and the University of Washington (UW) in Seattle. He became an Assistant Member/Professor at FHCRC and the UW in 1980. Dr. Green joined the faculty of the Department of Microbiology at Dartmouth Medical School in 1983. From 1992-2002 Dr. Green served as Director of the Immunology Program, including the Immunology and Cancer Immunotherapy Program of the Norris Cotton Cancer Center, before becoming Chair of the Microbiology and Immunology (M/I) Department in July, 2002. From January, 2008 through September 2010, he served a one term as Dean of Dartmouth Medical School before returning to chairmanship of the M/I Department, and Director of the Dartmouth Community Medical School.


Selected Publications:

 

O'Connor MA, Green WR
Use of IRF-3 and/or IRF-7 knockout mice to study viral pathogenesis: lessons from a murine retrovirus-induced AIDS model.
J Virol 2014 Feb; 88(4):2349-53
PMID: 24335302

O'Connor MA, Green WR
The role of indoleamine 2,3-dioxygenase in LP-BPM5 murine retroviral disease progression.
Virol J 2013 May 17; 10:154
PMID: 23680027

Green KA, Cook WJ, Green WR
Myeloid-derived suppressor cells in murine retrovirus-induced AIDS inhibit T- and B-cell responses in vitro that are used to define the immunodeficiency.
J Virol 2013 Feb; 87(4):2058-71
PMID: 23221564

Li W, Green WR
Immunotherapy of murine retrovirus-induced acquired immunodeficiency by CD4 T regulatory cell depletion and PD-1 blockade.
J Virol 2011 Dec; 85(24):13342-53
PMID: 21917983

Li W, Carlson TL, Green WR
Stimulation-dependent induction of CD154 on a subset of CD4+ FoxP3+ T-regulatory cells.
Int Immunopharmacol 2011 Sep; 11(9):1205-10
PMID: 21496498

Rutkowski MR, Stevens CA, Green WR
Impaired memory CD8 T cell responses against an immunodominant retroviral cryptic epitope.
Virology 2011 Apr 10; 412(2):256-68
PMID: 21295815

Carlson TL, Green KA, Green WR
Alternative translational reading frames as a novel source of epitopes for an expanded CD8 T-cell repertoire: use of a retroviral system to assess the translational requirements for CTL recognition and lysis.
Viral Immunol 2010 Dec; 23(6):577-83
PMID: 21142443

Rutkowski MR, Ho O, Green WR
Defining the mechanism(s) of protection by cytolytic CD8 T cells against a cryptic epitope derived from a retroviral alternative reading frame.
Virology 2009 Aug 1; 390(2):228-38
PMID: 19539970

Green KA, Okazaki T, Honjo T, Cook WJ, Green WR
The programmed death-1 and interleukin-10 pathways play a down-modulatory role in LP-BM5 retrovirus-induced murine immunodeficiency syndrome.
J Virol 2008 Mar; 82(5):2456-69
PMID: 18094175

Ho O, Green WR
Alternative translational products and cryptic T cell epitopes: expecting the unexpected.
J Immunol 2006 Dec 15; 177(12):8283-9
PMID: 17142722